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Table 4 Effects of culture conditions on chitinase production by strain TC-1

From: Characterization of a novel chitinolytic Serratia marcescens strain TC-1 with broad insecticidal spectrum

Items tested

Chitinase activity (U ml−1)

Carbon source

 

 Coloidal chitin

18.89 ± 0.04 Aa

 Chitin

12.69 ± 0.04 Bb

 Starch

3.09 ± 0.06 Cd

 Celullose

3.28 ± 0.03 Cc

Nitrogen source

 

 Peptone

17.8 ± 0.17 Aa

 Ammonium sulphate

9.49 ± 0.14 Cc

 Yeast extract

15.37 ± 0.25 Bb

 Beef extract

15.16 ± 0.19 Bb

Incubation time (h)

 

 24

7.75 ± 0.24 Gh

 30

8.2 ± 0.11 Fg

 36

8.45 ± 0.11 Fg

 42

9.98 ± 0.23 Ef

 48

10.44 ± 0.05 De

 54

12.81 ± 0.13 Cc

 60

17.44 ± 0.07 Aa

 66

14.69 ± 0.28 Bb

 72

12.47 ± 0.19 Cd

Incubation temperature (°C)

 

 24

10.55 ± 0.06 Cc

 26

14.91 ± 0.09 Bb

 28

17.33 ± 0.17 Aa

 30

14.92 ± 0.15 Bb

 32

9.69 ± 0.08 Cd

Colloidal concentration (g l-1)

 

 7.5

13.08 ± 0.25 Ee

 8.0

14.39 ± 0.16 Dd

 8.5

18.51 ± 0.09 Bb

 9.0

18.68 ± 0.08 Bb

 9.5

20.79 ± 0.19 Aa

 10.0

16.43 ± 0.10 Cc

 10.5

10.91 ± 0.10 Ff

  1. The analysis of variance for each item was independently tested. Data with different capitals and lowercases in the same item represent significant differences at P < 0.01 and P < 0.05 levels, respectively