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Fig. 5 | AMB Express

Fig. 5

From: A joint PCR-based gene-targeting method using electroporation in the pathogenic fungus Trichosporon asahii

Fig. 5

Generation of cnb1 gene-deficient mutant using DNA fragment produced by joint PCR. A DNA fragment 2 produced by joint PCR was added to competent T. asahii cells prepared by culture for 1 day and electroporated (time constant protocol: 1.8 kV, 5 ms). Colony PCR was performed on colonies (samples 1–15) grown on SDA containing nourseothricin (300 µg/ml). B Location of the primers for confirming the genome structure of the cnb1 gene-deficient candidate by PCR. C Confirmation of the cnb1 gene-deficiency of the cnb1 gene-deficient candidate by PCR using extracted genome DNA

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