Skip to main content
AMB Express
Fig. 2 | AMB Express

Fig. 2

From: A joint PCR-based gene-targeting method using electroporation in the pathogenic fungus Trichosporon asahii

Fig. 2

Effect of the length of homologous regions for gene transfer by electroporation in T. asahii. A Illustration of DNA fragments used in this study. B Electrophoresis of DNA fragments amplified by PCR. C The PCR-amplified 5ʹ-UTR (cnb1) -NAT1-3ʹ-UTR (cnb1) fragments were added to the competent T. asahii cells prepared by culture for 1 day and electroporated (time constant protocol: 1.8 kV, 5 ms). The number of colonies grown on SDA containing nourseothricin (300 µg/ml) was counted

Back to article page