Table 1 Bacterial Strains and Plasmids were used or developed in this study
Strain/Plasmid | Description | Reference |
|---|---|---|
Strains | ||
E. coli TOP10 | Fˊ(lacIq Tn10 (tetR)) mcrAD ΔlacX74 deoR nupG recA1 araD139Δ(ara-leu)7697 galU galK rpsL (strR) endA1λ- cloning host | Invitrogen |
B. subtilis 168 | trpC2 (ATCC 33,712, (ATCC, Manassas, VA, USA) | (Harwood 1992) |
RBSFA | B. subtilis 168 carrying pDG148-hasA | Developed in this study |
RBSTr3 | B. subtilis 168 carrying pDG148Tr3 | Developed in this study |
RBSTr4 | B. subtilis 168 carrying pDG148Tr4 | Developed in this study |
Plasmids | ||
pDG148 | Multicopy B. subtilis and E. coli shuttle vector; Apr, Kmr, replicative | (Stragier et al. 1988) |
pDG148-hasA | pDG148 carrying hasA gene | Developed in this study |
pDG148-Tr3 | pDG148 carrying Tr3 gene* | Developed in this study |
pDG148-Tr4 | pDG148 carrying Tr4 gene* | Developed in this study |