Fig. 8From: Preparation and identification of a single domain antibody specific for adenovirus vectors and its application to the immunoaffinity purification of adenovirusesBinding activity and specificity were analyzed using indirect ELISA. The recombinant sdAbs 5 and 31 showed strong binding activity to human adenovirus Ad5, rAd26, and ChAd63 viruses in ELISAs at a concentration of 5 μg/ml. Protein extracts from E. coli transformed with the empty pET-25b ( +) vector were used as a negative control, and PBS solution was used as a blank controlBack to article page