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AMB Express
Fig. 7 | AMB Express

Fig. 7

From: Preparation and identification of a single domain antibody specific for adenovirus vectors and its application to the immunoaffinity purification of adenoviruses

Fig. 7

Expression and purification of the recombinant sdAbs. The plasmids of sdAb 5 and sdAb 31 from phage ELISA were isolated, and the VHH gene was digested with NcoI and NotI and ligated to pET-25b ( +) –SBP. The SDS-PAGE results showed that the recombinant sdAb 5 and sdAb 31 antibodies were expressed in a soluble form with an expected molecular weight of 20 kDa and could be successfully purified from Ni–NTA agarose

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