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AMB Express
Fig. 4 | AMB Express

Fig. 4

From: Preparation and identification of a single domain antibody specific for adenovirus vectors and its application to the immunoaffinity purification of adenoviruses

Fig. 4

Identification of phage display antibody library. The electro-transformed TG1 bacterial solution from 101 to 1010 with fresh 2 × YT/AG media was grown at 37 °C. The next day, the calculated volume of the phage antibody library was 1.8 × 109. Twenty-four clones were randomly selected from the 2 × YT/AG plate for colony PCR identification. After 1% agarose gel electrophoresis, 22 clones with amplicons of 600 bp fragments were obtained, and we calculated that the recombination rate of the antibody library was 91.6%

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