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AMB Express
Fig. 3 | AMB Express

Fig. 3

From: Preparation and identification of a single domain antibody specific for adenovirus vectors and its application to the immunoaffinity purification of adenoviruses

Fig. 3

Amplification of VHH gene. cDNA was used as a template, and a fragment containing the leader sequence to the CH2 region of the IgG (900 bp for VH and 600 bp for VHH) was amplified with primers P1 and P2. The result is shown in Fig. 3(a). The 600 bp fragment was purified using 1% agarose gel electrophoresis and used as the template for the second round of PCR with primers P3 and P4, which anneal to the VHH FR1 and FR4 regions, respectively. The target bands of approximately 400 bp were successfully amplified, as shown in Fig. 3(b)

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