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AMB Express
Fig. 12 | AMB Express

Fig. 12

From: Preparation and identification of a single domain antibody specific for adenovirus vectors and its application to the immunoaffinity purification of adenoviruses

Fig. 12

Immunoaffinity purification of adenovirus. The bound viruses were eluted with 2 M, 4 M NaCl solution and PBS, and the eluent was added to the cultured HEK293A cells for reinfection. A blank and negative control group were established. After inoculation, the cells were placed at 37 °C in a 5% CO2 incubator for 24 h to 48 h and observed and counted. A 2 M eluent group; B 4 M eluent group; C blank control group; D PBS buffer group. Green fluorescence was detected in the 2-M and 4-M NaCl solution eluent-infected cells, as shown in A and B, but we did not detect green fluorescence in PBS eluent-infected cells or non-infected cells, as shown in C and D. These results indicate that the immobilized sdAbs specifically bind to the adenoviruses and isolate them from the culture medium

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