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Table 1 RNA purification from a covid-9 positive sputum sample

From: In-house protocol: spin-based viral RNA purification

 

Concentration (ng/µl)

Purity (260/280 ratio)

A RNA purification data

 QIagen extraction kit

20.9

1.98

 In-house solutions

19.8

2.02

 

N-gene CT

N-gene viral load

Copies/ml

E-gene CT

E-gene viral load

Copies/ml

RNaseP

CT

Positive/negative

B qRT-PCR results

 QIagen extraction kit

20.83

1.6 × 105

23.92

7.13 × 103

26.96

Positive

 In-house solutions

21.9

6.9 × 104

24.67

4.16 × 103

27.3

Positive

 Positive control

19.4

4.9 × 105

24.52

4.6 × 103

28.59

Positive

 Negative control

U.D

U.D

U.D

U.D

U.D

Negative