Fig. 3

Evaluation of LPA carrier versus Qiagen carrier. RNA was purified from infected Vero cells with measles virus. The same amount of cells used as startup point to compare Qiagen carrier and LPA carrier using all Qiagen reagents (i.e. the only difference between the tubes is type of carrier). A RT-PCR of N-450 using MeV210 and MeV217 primers for RNA purified with Qiagen reagents and LPA carrier. The samples were compared to whole Qiagen setting control with their carrier by running on 2%Agarose with histogram showing percentage of bands intensities comparable to Qiagen kit. B RT-PCR of N-450 using MeV210 and MeV217 primers for RNA purified with in-house protocol comparable to Qiagen QIAamp viral RNA mini kit