Fig.5
From: Callose deposited at soybean sieve element inhibits long-distance transport of Soybean mosaic virus
Detection of SMV-CP gene product. A Detection of SMV CP gene on grafted plants; M: DL 2000 bp DNA marker; 1: positive control (Jihuang 13 leaves inoculated with SC-8); 2: negative control (simulating inoculated leaves); 3: scion nf58 leaves after inoculation; 4: Stem of rootstock above the grafting site; 5: Stem of rootstock below the grafting site; 6: non-inoculated upper leaves of rootstock. B SMV-CP gene detection in rootstock when scion nf58 inoculated with N3 was pre-treated with 2-DDG. M:DL2000 bp DNA marker; 1: positive control (Jihuang 13 leaves inoculated with SC-8); 2: negative control (leaves inoculated with water); 3: upper leaves of Wuxing 2; 4: stem of rootstock above the grafting site; 5: stem of rootstock below the grafting site; 6: scion leaves after inoculation. C Western blotting detection of SMV CP. M: Easysee western marker; 1: Leaves of scion after inoculation with SMV; 2: Rootstock stem below the grafting site; 3: Uninoculated rootstock leaves above the grafting site; 4: Stem of rootstock above grafting site