Table 1 Primers for amplification of predicted and 16S rRNA genes
From: Whole genome sequencing and analysis of fenvalerate degrading bacteria Citrobacter freundii CD-9
Target fragments | Forward primer sequence (5ʹ → 3ʹ) | Reverse primer sequence (5ʹ → 3ʹ) | Function annotation |
|---|---|---|---|
EstP1 | GTGAAGCCGTGGCGTTGT | GTGCGGTTTGCTGTAGGG | Beta-phosphoglucomutase |
EstP2 | ACGCCACAAACCTGCTCC | CGCCGCCTGTATTCCAAA | Beta-phosphoglucomutase |
EstP3 | TCCAGGCGTTTGGCATTA | CGACACCGTGCTCTTCGT | Beta-phosphoglucomutase |
EstP4 | ATCAGGTCGGCGAGTATTT | GCGGTTATCCCAGTTGTT | Glucose-6-phosphate 1-dehydrogenase |
EstP5 | AGCCTTACGGTACGACTTCC | ACGCCGCTTTGTTTCTGC | Putative hydrolase |
EstP6 | TATGGCTACAACCGTCCTG | ATCACCGTGGATCACCAG | Pimeloyl- [acyl-carrier protein] methyl ester esterase |
EstP7 | GAGGCGTTGTTTGAGGCA | CAGGGTCTGGGCTTTGATT | 2-Hydroxy-6-ketonona-2,4-dienedioic acid hydrolase |
EstP8 | CTGGCGACTATTCCCTCAA | CGTAAAGCCCATACCACAAC | Monoterpene epsilon-lactone hydrolase |
EstP9 | GTCGATCTGCGAGAACCG | CCGCCTTAGCAAACACCA | Pimeloyl- [acyl-carrier protein] methyl ester esterase |
EstP10 | GCGTGCTGGTGCTGGAAA | GACTGGTTGTGGCGGTGA | Gentisate 1,2-dioxygenase |
EstP11 | TTCTGTTTAGGCGTGGGAGC | CATGTTGTAGGACACGGCAAG | 2,3-Dihydroxyphenylpropionate 1,2-dioxygenase |
EstP12 | CTGCTGGAATTTCCGTTTG | GCATTGCCCATTGCTGTT | 4-Carboxymuconolactone decarboxylase |
EstP13 | GTCGGCATTTATCGTATGG | GTGATGGCAATCGGAAGA | 4-Hydroxybenzoate decarboxylase |
16S rRNA | CGCTACCATGCAGTCGAACG | GGTCCCCCTCTTTGGTCTTG | – |