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Table 4 Test performance of the multiplex polymerase chain reaction (M-PCR) and loop mediated isothermal amplification (LAMP) in detection of Clostridioides difficile isolates compared to the culture method

From: Loop mediated isothermal amplification of Clostridioides difficile isolates in gastrointestinal patients

 

Culture for Clostridioides difficile

Positive

Negative

Total

M-PCR Positive

10 (20.0%)

7 (14.0%)

17 (34.0%)

M-PCR Negative

0 (0.0%)

33 (66.0%)

33 (6.0%)

Total

10 (20.0%)

40 (80.0%)

50 (100.0%)

Sensitivity (%) (95% CI)

100.0% (69.2 to 100.0%)

Specificity (%) (95% CI)

82.5% (67.2 to 92.7%)

Positive predictive value (%) (95% CI)

58.8% (42.2 to 73.7%)

Negative predictive value (%) (95% CI)

100.0%

Test accuracy (%) (95% CI)

86.0% (73.3 to 94.2%)

Kappa coefficient (κ)

0.7

LAMP Positive

10 (20.0%)

5 (10.0%)

15 (30.0%)

LAMP Negative

0 (0.0%)

35 (70.0%)

35 (70.0%)

Total

10 (20.0%)

40 (80.0%)

50 (100.0%)

Sensitivity (%) (95% CI)

100.0% (69.2 to 100.0%)

Specificity (%) (95% CI)

87.5% (73.2 to 95.8%)

Positive predictive value (%) (95% CI)

66.7% (46.8 to 82.0%)

Negative predictive value (%) (95% CI)

100.0%

Test accuracy (%) (95% CI)

90.0% (78.2 to 96.7%)

Kappa coefficient (κ)

0.7