Fig. 3From: Broad thermal spectrum metagenomic laccase with action for dye decolorization and fentin hydroxide treatmentEffects of pH and temperature on LacMeta activity. a Effect of pH on laccase activity. The buffers used were: 20 mM sodium citrate (pH 3.5 to 6.5) (black circle), 20 mM sodium phosphate (pH 6.5 to 7.0) (white triangle); 20 mM TRIS–HCl (pH 7.0 to 7.5 and 8.5 to 9.0) (black square), 20 mM HEPES (pH 7.5 to 8.5) (white rhombus) and sodium bicarbonate at 20 mM (9.0 to 11) (white circle). b Effect of temperature on activity. An enzymatic activity was measured from 4 to 60 °C in 20 mM sodium citrate buffer (pH 4.0). The tests were performed with 3 replicates. The values followed by the same letter do not differ statistically, according to the ANOVA and Tukey test, with a 5% probabilityBack to article page