Fig. 2

LacMeta expression and purification. m) Molecular mass marker. a 10% SDS-PAGE metal affinity purification fraction of the recombinant protein. b Native LacMeta gel stained with 1 mM of 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), ABTS, in sodium citrate buffer pH 4.0. c Chromatographic profile of the gel filtration purified metagenomic. The internal image corresponds enzyme equivalent fractions purified laccase (2–8). Molecular mass estimation of LacMeta by gel filtration based on linear correlation. Elution of standard molecular mass of LacMeta and protein standards versus their logarithmic molecular mass values. P1–P4: Proteins used as molecular mass standards: ρ-amino benzoic acid (pABA) (0.13 kDa), Ribonuclease A (13.7 kDa), albumin (43 kDa), (Bovine thyroglobulin (670 kDa), γ-globulin (150 kDa), Protein Standard Mix 15 ± 600 kDa, Sigma, St. Louis, MO, USA)