Target genes | Primers sequences | Amplicon size (bp) | Reaction volume (µL) | Amplification (35 cycles) | References | ||
---|---|---|---|---|---|---|---|
Denat | Annealing | Extension | |||||
eaeA (Intimin) | ATGCTTAGTGCTGGTTTAGG GCCTTCATCATTTCGCTTTC | 248 | 25 | 94Â ËšC 30Â s | 51Â ËšC 30Â s | 72Â ËšC 30Â s | (Bisi-Johnson et al. 2011) |
hlyA (Hemolysin) | AACAAGGATAAGCACTGTTCTGGCT ACCATATAAGCGGTCATTCCCGTCA | 1177 | 25 | 94Â ËšC 30Â s | 54Â ËšC 40Â s | 72Â ËšC 45Â s | (Piva et al. 2003) |
stx1 (Shiga-toxin 1) | ACACTGGATGATCTCAGTGG CTGAATCCCCCTCCATTATG | 614 | 50 | 94Â ËšC 30Â s | 58Â ËšC 40Â s | 72Â ËšC 45Â s | (Dipineto et al. 2006) |
stx2 (Shiga-toxin 2) | CCATGACAACGGACAGCAGTT CCTGTCAACTGAGCAGCACTTTG | 779 | |||||
sul1 (Sulfonamides resistance) | CGGCGTGGGCTACCTGAACG GCCGATCGCGTGAAGTTCCG | 433 | 25 | 94Â ËšC 30Â s | 55Â ËšC 45Â s | 72Â ËšC 45Â s | (Ibekwe et al. 2011) |
tetA (Tetracycline resistance) | GGTTCACTCGAACGACGTCA CTGTCCGACAAGTTGCATGA | 576 | 50 | 94Â ËšC 30Â s | 55Â ËšC 40Â s | 72Â ËšC 45Â s | (Randall 2004) |
tetB (Tetracycline resistance) | CCTCAGCTTCTCAACGCGTG GCACCTTGCTCATGACTCTT | 634 | |||||
qnrA (Quinolones resistance) | AGAGGATTTCTCACGCCAGG TGCCAGGCACAGATCTTGAC | 580 | 50 | 95Â ËšC 1Â min | 54Â ËšC 1Â min | 72Â ËšC 1Â min | (Cattoir et al. 2007) |
qnrB (Quinolones resistance) | GGMATHGAAATTCGCCACTG TTTGCYGYYCGCCAGTCGAA | 264 | |||||
qnrS (Quinolones resistance) | GCAAGTTCATTGAACAGGGT TCTAAACCGTCGAGTTCGGCG | 428 | |||||
blaCTX-M (Cephalosporins resistance) | ATGTGCAGYACCAGTAARGTKATGGC TGGGTRAARTARGTSACCAGAAYCAGCGG | 593 | 25 | 94Â ËšC 30Â s | 54Â ËšC 40Â s | 72Â ËšC 45Â s | (Archambault et al. 2006) |
blaTEM (Penicillin resistance) | ATCAGCAATAAACCAGC CCCCGAAGAACGTTTTC | 516 | 25 | 94Â ËšC 30Â s | 54Â ËšC 40Â s | 72Â ËšC 45Â s | (Colom et al. 2003) |
blaKPC (Carbpenem resistance) | ATGTCACTGTATCGCCGTCT TTACTGCCCGTTGACGCCC | 892 | 25 | 94Â ËšC 1Â min | 55Â ËšC 1Â min | 72Â ËšC 1Â min | (Xia et al. 2012) |
blaNDM-1 (Carbpenem resistance) | GGCGGAATGGCTCATCACGA CGCAACACAGCCTGACTTTC | 287 | 25 | 94Â ËšC 30Â s | 55Â ËšC 30Â s | 72Â ËšC 30Â s |