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AMB Express

Table 2 Experimental ‘OVAT’ data and FFNN predicted data for AlkB specific activity

From: Enhanced production of alkane hydroxylase from Penicillium chrysogenum SNP5 (MTCC13144) through feed-forward neural network and genetic algorithm

S. no.

V1

V2

V3

V4

V5

V6

Experimental AlkB specific activity (U/mg)

Predicted AlkB specific activity (U/mg)

Error

1

0.25

28

7

9

1

0.1

64.8

57.17

7.62

2

0.5

28

7

9

1

0.1

74.53

69.17

5.35

3

0.75

28

7

9

1

0.1

88.38

84.82

3.55

4

1

28

7

9

1

0.1

97.41

100.50

− 3.09

5

1.25

28

7

9

1

0.1

100.45

112.56

− 12.1

6

1.5

28

7

9

1

0.1

117.93

119.80

− 1.87

7

1.75

28

7

9

1

0.1

114.43

123.10

− 8.67

8

2

28

7

9

1

0.1

112.64

123.85

− 11.2

9

2.25

28

7

9

1

0.1

112.79

123.14

− 10.3

10

2.5

28

7

9

1

0.1

112.51

121.64

− 9.13

11

2.75

28

7

9

1

0.1

112.12

119.70

− 7.58

12

3

28

7

9

1

0.1

111.77

117.50

− 5.73

13

3.25

28

7

9

1

0.1

111.56

115.11

− 3.55

14

3.5

28

7

9

1

0.1

111.66

112.55

− 0.89

15

3.75

28

7

9

1

0.1

110.97

109.79

1.17

16

4

28

7

9

1

0.1

110.39

106.78

3.60

17

1.5

20

7

9

1

0.1

75.52

78.51

− 2.99

18

1.5

21

7

9

1

0.1

80.33

79.05

1.27

19

1.5

22

7

9

1

0.1

84.23

80.77

3.45

20

1.5

23

7

9

1

0.1

87.65

84.76

2.88

21

1.5

24

7

9

1

0.1

93.66

93.06

0.59

22

1.5

25

7

9

1

0.1

100.71

108.41

− 7.70

23

1.5

26

7

9

1

0.1

120.44

130.55

− 10.11

24

1.5

27

7

9

1

0.1

142.25

140.41

1.83

25

1.5

28

7

9

1

0.1

140.44

119.80

20.63

26

1.5

29

7

9

1

0.1

138.33

122.19

16.13

27

1.5

30

7

9

1

0.1

137.13

133.15

3.976

28

1.5

31

7

9

1

0.1

136.77

135.37

1.39

29

1.5

32

7

9

1

0.1

135.56

129.16

6.39

30

1.5

33

7

9

1

0.1

123.44

117.46

5.97

31

1.5

34

7

9

1

0.1

96.46

102.78

− 6.32

32

1.5

35

7

9

1

0.1

93.22

86.96

6.25

33

1.5

36

7

9

1

0.1

60.6

71.36

− 10.76

34

1.5

37

7

9

1

0.1

42.55

56.90

− 14.35

35

1.5

38

7

9

1

0.1

42.02

44.13

− 2.11

36

1.5

27

4

9

1

0.1

39.46

34.51

4.94

37

1.5

27

4.25

9

1

0.1

40.13

40.10

0.021

38

1.5

27

4.5

9

1

0.1

41.9

45.22

− 3.32

39

1.5

27

4.75

9

1

0.1

43.55

49.65

− 6.10

40

1.5

27

5

9

1

0.1

53.46

53.34

0.11

41

1.5

27

5.25

9

1

0.1

56.67

56.43

0.23

42

1.5

27

5.5

9

1

0.1

66.75

59.41

7.33

43

1.5

27

5.75

9

1

0.1

67.89

63.67

4.21

44

1.5

27

6

9

1

0.1

73.71

72.40

1.30

45

1.5

27

6.25

9

1

0.1

87.43

90.08

− 2.65

46

1.5

27

6.5

9

1

0.1

110.85

114.85

− 4.00

47

1.5

27

6.75

9

1

0.1

132.23

134.05

− 1.82

48

1.5

27

7

9

1

0.1

148.27

140.41

7.85

49

1.5

27

7.25

9

1

0.1

137.76

137.13

0.62

50

1.5

27

7.5

9

1

0.1

135.68

128.47

7.20

51

1.5

27

7.75

9

1

0.1

121.3

116.76

4.53

52

1.5

27

8

9

1

0.1

104.81

103.18

1.62

53

1.5

27

8.25

9

1

0.1

79.55

88.59

− 9.04

54

1.5

27

8.5

9

1

0.1

69.19

73.74

− 4.55

55

1.5

27

8.75

9

1

0.1

43.66

59.37

− 15.71

56

1.5

27

9

9

1

0.1

39.44

46.18

− 6.74

57

1.5

27

7

5

1

0.1

80.56

89.64

− 9.08

58

1.5

27

7

5.5

1

0.1

96.34

94.52

1.81

59

1.5

27

7

6

1

0.1

99.39

99.97

− 0.58

60

1.5

27

7

6.5

1

0.1

100.34

105.99

− 5.65

61

1.5

27

7

7

1

0.1

115.79

112.58

3.25

62

1.5

27

7

7.5

1

0.1

120.93

119.47

1.45

63

1.5

27

7

8

1

0.1

139.98

126.62

13.35

64

1.5

27

7

8.5

1

0.1

141.32

133.71

7.605

65

1.5

27

7

9

1

0.1

143.06

140.41

2.64

66

1.5

27

7

9.5

1

0.1

144.12

146.32

− 2.20

67

1.5

27

7

10

1

0.1

144.5

151.07

− 6.57

68

1.5

27

7

10.5

1

0.1

142.11

154.29

− 12.19

69

1.5

27

7

11

1

0.1

148.6

155.75

− 7.15

70

1.5

27

7

11.5

1

0.1

148.93

155.37

− 6.44

71

1.5

27

7

12

1

0.1

155.5

153.30

2.19

72

1.5

27

7

12.5

1

0.1

149.45

149.97

− 0.52

73

1.5

27

7

13

1

0.1

138.5

146.00

− 7.50

74

1.5

27

7

13.5

1

0.1

136.89

142.21

− 5.32

75

1.5

27

7

14

1

0.1

136.96

139.58

− 2.62

76

1.5

27

7

12

0.25

0.1

133.65

130.72

2.92

77

1.5

27

7

12

0.5

0.1

146.44

139.22

7.21

78

1.5

27

7

12

0.75

0.1

147.66

147.40

0.253

79

1.5

27

7

12

1

0.1

150.2

153.30

− 3.10

80

1.5

27

7

12

1.25

0.1

155.65

155.77

− 0.126

81

1.5

27

7

12

1.5

0.1

162.25

155.13

7.119

82

1.5

27

7

12

1.75

0.1

161.44

153.36

8.07

83

1.5

27

7

12

2

0.1

160.94

153.25

7.68

84

1.5

27

7

12

2.25

0.1

160.11

154.93

5.17

85

1.5

27

7

12

2.5

0.1

160.33

154.35

5.97

86

1.5

27

7

12

2.75

0.1

158.45

149.29

9.15

87

1.5

27

7

12

3

0.1

150.77

141.48

9.28

88

1.5

27

7

12

1.5

0.05

147.44

148.59

− 1.15

89

1.5

27

7

12

1.5

0.15

158.33

160.52

− 2.19

90

1.5

27

7

12

1.5

0.1

164.06

155.13

8.92

91

1.5

27

7

12

1.5

0.25

164.33

172.13

− 7.80

92

1.5

27

7

12

1.5

0.2

165.1

165.91

− 0.81

93

1.5

27

7

12

1.5

0.35

169.43

185.65

− 16.2

94

1.5

27

7

12

1.5

0.3

185.38

179.12

6.25

95

1.5

27

7

12

1.5

0.45

184.32

191.79

− 7.47

96

1.5

27

7

12

1.5

0.4

198.94

190.09

8.84

97

1.5

27

7

12

1.5

0.5

198

191.66

6.33

98

1.5

27

7

12

1.5

0.55

197.37

191.21

6.15

99

1.5

27

7

12

1.5

0.6

192.69

191.37

1.31

100

1.5

27

7

12

1.5

0.65

193.44

192.27

1.160

101

1.5

27

7

12

1.5

0.7

195.56

193.77

1.78

102

1.5

27

7

12

1.5

0.75

194.33

195.68

− 1.35

103

1.5

27

7

12

1.5

0.8

197.06

197.92

− 0.86

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