Characterization, antioxidant and anticoagulant properties of exopolysaccharide from marine microalgae

AMB Express

Table 4 Analysis of anticoagulant activity by aPTT and PT assay on Sps

	µg/mL	aPTT(s)	T₁/T₀^a	µg/mL	PT(s)	T₁/T₀^a
Chlorella sp. (N₄)	50	24.19 ± 0.81	0.71 ± 0.03	25	12.88 ± 0.02	0.98 ± 0.008
	75	23.83 ± 0.31	0.70 ± 0.009	50	12.89 ± 0.14	0.98 ± 0.005
	100	38.56 ± 1.99	1.18 ± 0	75	12.90 ± 0.09	0.99 ± 0.003
	200	19.41 ± 2.19	0.57 ± 00.01	100	13.04 ± 0.007	1 ± 0.0002
	–		–	200	14.52 ± 0.5	1.11 ± 0.01
Chlorella sorokiniana	50	26.79 ± 1.28	0.79 ± 0.03	25	11.90 ± 0.28	0.91 ± 0.01
	75	23.08 ± 0.55	0.68 ± 0.01	50	12.96 ± 0.07	0.99 ± 0.002
	100	35.83 ± 1.06	1.05 ± 0.03	75	13.3 ± 0.82	1.05 ± 0.004
	200	32.56 ± 2.19	0.98 ± 0.03	100	13.01 ± 0.04	0.99 ± 0.001
	–		–	200	14.26 ± 0.34	1.09 ± 0.01
Chlorella sp. (L₂)	50	23.41 ± 0.94	0.69 ± 0.02	25	11.88 ± 0.16	0.91 ± 0.006
	75	16.53 ± 1.45	0.48 ± 0.04	50	12.91 ± 0.09	0.99 ± 0.003
	100	36.78 ± 1.24	1.08 ± 0.03	75	12.54 ± 0.23	0.96 ± 0.008
	200	18.65 ± 0.55	0.55 ± 0.01	100	13.56 ± 0.27	1.04 ± 0.01
	–		–	200	13 ± 0.07	0.99 ± 0.002
Chlorella sp. (D₁)	50	19.69 ± 0.01	0.58 ± 0.006	25	12.45 ± 0.44	0.95 ± 0.01
	75	27.89 ± 0.43	0.82 ± 0.01	50	13.75 ± 0.54	1.07 ± 0.03
	100	32.05 ± 1.20	0.94 ± 0.03	75	12.70 ± 0.36	0.97 ± 0.01
	200	33.74 ± 0.68	0.99 ± 0.02	100	13.43 ± 0.08	0.99 ± 0.03
	–		–	200	13.82 ± 0.53	1.06 ± 0.02
Picochlorum sp.	50	31.69 ± 1.87	0.93 ± 0.05	25	12.36 ± 0.11	0.94 ± 0.004
	75	21.3 ± 1.20	0.62 ± 0.03	50	11.26 ± 0.53	0.86 ± 0.02
	100	25.07 ± 0.47	0.74 ± 0.01	75	12.85 ± 0.21	0.98 ± 0.008
	200	21.49 ± 0.51	0.63 ± 0.01	100	12.62 ± 0.49	0.96 ± 0.01
	–		–	200	13.54 ± 0.06	1.03 ± 0.022

HEP 5 µg/mL; aPTT: 60.05 ± 0.007 s; Control: 33.87 ± 0.45 s and HEP 25 µg/mL; PT: 13.69 ± 0.2 s; Control: 13.03 ± 0.2 s. Data expressed as mean ± standard deviation (n = 3)
^aClotting time in presence sPS(T₁) and absence (T₀)