Fig. 5

DM miniprep plasmids can efficiently be used in DNA quality-sensitive applications. A The transfection efficiency of PC3 prostate cancer cells with the DM (DM) minipreps plasmid was similar to that of the ZymoPURE-EndoZero Midiprep (Endo) and QIAGEN Midi (Midi) Kit-derived results. One of three independent experiments is reported. B pcDNA 3.1/His/LacZ plasmid purified with the DM minipreps method forms GFP-TAT-fusion protein complexes. pcDNA 3.1/His/LacZ plasmid was premixed with various amounts of the GFP-TAT fusion protein as described in methods. Samples were resolved by agarose gel electrophoresis. GFPmodTAT and GFP written on the right correspond to pure proteins.