Fig. 4
DM minipreps plasmids have a lower amount of host genomic DNA. A The amount of host genomic DNA in DM and SM plasmids; lanes 1–4—reference interval of quantities’ DNA for semiquantitative analysis; P, E, L—pBR322, pEGFP, LacZ plasmids purified by DM at oscillation amplitude 45° and 3, 4, 5—300, 400, 500 rpm oscillation frequency; SMP, SME, SML—pBR322, pEGFP, LacZ plasmids purified by SM method. B Densitometry analysis of amplified products is presented. C The amount of host genomic DNA in pEGFP plasmid DNA purified either by SM (S) or DM (D) minipreps and the ZymoPURE-EndoZero (Endo) Midiprep or QIAGEN Midi kit (Midi). D Densitometry analysis of amplified products is presented. Assays were performed in triplicate, and error bars represent the standard deviation