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Table 3 Composition of the real-time fluorescence LAMP reaction mix

From: Evaluation of the real-time fluorescence loop-mediated isothermal amplification assay for the detection of Ureaplasma urealyticum

  Components Volume
Reaction mix 20 mM Tris–HCl (pH 8.8) 12.5 μL
  10 mM KCl  
  8 mM MgSO4  
  10 mM (NH4)2SO4  
  0.1% Tween-20  
  1 mM Betaine  
  1.6 mM dNTP  
Primer mix FIP (1.6 μM) and BIP (1.6 μM) 1 μL
  F3 (0.2 μM) and B3 (0.2 μM)  
  FLF (0.8 μM) and FLB (0.8 μM)  
Nuclease-free water   8 μL
Bst DNA polymerase   1 μL
SYTO-9   0.5 μL
DNA template   2 μL
Total volume   25 μL
  1. Reaction condition: 63 °C, 45–60 min