Table 3 Composition of the real-time fluorescence LAMP reaction mix
Components | Volume | |
|---|---|---|
Reaction mix | 20 mM Tris–HCl (pH 8.8) | 12.5 μL |
10 mM KCl | ||
8 mM MgSO4 | ||
10 mM (NH4)2SO4 | ||
0.1% Tween-20 | ||
1 mM Betaine | ||
1.6 mM dNTP | ||
Primer mix | FIP (1.6 μM) and BIP (1.6 μM) | 1 μL |
F3 (0.2 μM) and B3 (0.2 μM) | ||
FLF (0.8 μM) and FLB (0.8 μM) | ||
Nuclease-free water | 8 μL | |
Bst DNA polymerase | 1 μL | |
SYTO-9 | 0.5 μL | |
DNA template | 2 μL | |
Total volume | 25 μL |