Table 2 Primers used for the real-time fluorescence LAMP and PCR reaction system
UreaseB-1 Primers | Sequence (5ʹ–3ʹ) | Length (bp) |
|---|---|---|
F3 | AGGAGATAATGATTATATGTCAGGA | 25 |
B3 | TAACGCTATCACCAGTTGTG | 20 |
FIP(F1c + F2) | CAACTTGGATAGGACGGTCACCAATTAGTACCAGGAGCAATTAACT | 46 |
BIP(B1c + B2) | ATTCCATCAGGTACTGCTATTCGTTTTCCGTTAACTAAGCCGTT | 44 |
LOOPF | TCTCTACCTTCGTTCATCACAATT | 24 |
LOOPB | TTAGTCGGAACACGTGAAGTT | 21 |
UreaseB-2 Primers | Sequence (5ʹ–3ʹ) | Length (bp) |
|---|---|---|
F3 | AGGAGATAATGATTATATGTCAGGA | 25 |
B3 | CACCAGTTGTGATACCATATAGAT | 24 |
FIP(F1c + F2) | CAACTTGGATAGGACGGTCACCAATTAGTACCAGGAGCAATTAACT | 46 |
BIP(B1c + B2) | ATTCCATCAGGTACTGCTATTCGTTTTCCGTTAACTAAGCCGTT | 44 |
LOOPF | TCTCTACCTTCGTTCATCACAATT | 24 |
LOOPB | TTAGTCGGAACACGTGAAGTT | 21 |
UU PCR primer | Sequence (5ʹ–3ʹ) | Length (bp) |
|---|---|---|
UU forward | CAGGATCATCAAATCAATTCAC | 22 |
UU reverse | CATAATGTTCCCCTTCGTCTA | 21 |