Fig. 5

a Binding assay of DENV-LPs/1CprME, /1prME, /4CprME, and /4prME to heparin using ELISA. Heparin (1.8 ng) was coated onto each well of an ELISA plate, and each amount of purified protein was used for this ELISA, which was carried out according to the protocol described in the Materials and methods (Welch t-test, *p < 0.05, **p < 0.01, ***p < 0.001). b–e The binding activities between DENV-LPs/1CprME, /1prME, /4CprME, and /4prME toward heparin were examined by ITC