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Table 1 Purification of native enzymes from the cell-free broth of Geomyces sp. B10I. The activity was measured in a turbidimetric assay with PBSA as a substrate for enzyme activity. Unsuccessfully, it was not possible to determine the activity of purified enzymes after the IEC procedure. The assays were performed in duplicate

From: Identification of novel extracellular putative chitinase and hydrolase from Geomyces sp. B10I with the biodegradation activity towards polyesters

  Volume (mL) Protein (µg/mL) Activity (U/mL) Total protein (mg) Total activity (U) Specific activity (U/mg) Purification factor Yield (%)
Free-cell broth 350.0 77.31 ± 0.71 4.93 ± 0.01 27.06 ± 0.25 1.725.5 ± 4.95 63.77 ± 0.77 1 100
Concentrated free-cell broth 158.0 139.16 ± 2.56 10.76 ± 0.14 21.99 ± 0.4 1.700.08 ± 22.34 77.32 ± 0.40 1.21 98.52
60% (NH4)2SO4 11.0 52.47 ± 2.67 54.82 ± 2.8 0.66 ± 0.03 592.02 ± 15.24 903.22 ± 22.71 14.16 34.31