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Table 1 Purification of native enzymes from the cell-free broth of Geomyces sp. B10I. The activity was measured in a turbidimetric assay with PBSA as a substrate for enzyme activity. Unsuccessfully, it was not possible to determine the activity of purified enzymes after the IEC procedure. The assays were performed in duplicate

From: Identification of novel extracellular putative chitinase and hydrolase from Geomyces sp. B10I with the biodegradation activity towards polyesters

 

Volume (mL)

Protein (µg/mL)

Activity (U/mL)

Total protein (mg)

Total activity (U)

Specific activity (U/mg)

Purification factor

Yield (%)

Free-cell broth

350.0

77.31 ± 0.71

4.93 ± 0.01

27.06 ± 0.25

1.725.5 ± 4.95

63.77 ± 0.77

1

100

Concentrated free-cell broth

158.0

139.16 ± 2.56

10.76 ± 0.14

21.99 ± 0.4

1.700.08 ± 22.34

77.32 ± 0.40

1.21

98.52

60% (NH4)2SO4

11.0

52.47 ± 2.67

54.82 ± 2.8

0.66 ± 0.03

592.02 ± 15.24

903.22 ± 22.71

14.16

34.31