Sodium nitrate has no detrimental effect on milk fatty acid profile and rumen bacterial population in water buffaloes

AMB Express

Table 2 PCR primers for real time PCR

Target species	Forward/reverse	Primer sequences (5′– > 3′)	References
Total bacteria	F	CGGCAACGAGCGCAACCC	Denman and McSweeney 2006
Total bacteria	R	CCATTGTAGCACGTGTGTAGCC	Denman and McSweeney 2006
Methanogens	F	TTCGGTGGATCDCARAGRGC	Denman et al. 2007
Methanogens	R	GBARGTCGWAWCCGTAGAATCC	Denman et al. 2007
Protozoal	F	GCTTTCGWTGGATGTGTATT	Sylvester et al. 2004
Protozoal	R	CTTGCCCTCYAATCGTWCT	Sylvester et al. 2004
Fungi	F	GAGGAAGTAAAAGTCGTAACAAGGTTTC	Denman and McSweeney 2006
Fungi	R	CAAATTCACAAAGGGTAGGATGATT	Denman and McSweeney 2006
B. proteoclasticus	CprF	TCCGGTGGTATGAGATGGGC	Shingfield et al. 2012
	CprR	GTCGCTGCATCAGAGTTTCCT
	CprP	CCGCTTGGCCGTCCGACCTCTCAGTCCGAGCGG
B. fibrisolvens + Pseudobutyrivibrio spp.	BfiF	GCCTCAGCGTCAGTAATCG	Shingfield et al. 2012
B. fibrisolvens + Pseudobutyrivibrio spp.	BfiR	GGAGCGTAGGCGGTTTTAC	Shingfield et al. 2012
‘Atypical’ Butyrivibrio	AtbF	GACGGTGTATCAAGTCTGAAGTG	Shingfield et al. 2012
‘Atypical’ Butyrivibrio	AtbR	GCCGGCACTGAAAGACTATGTC	Shingfield et al. 2012
B. hungatei	BhuF	AGGGTAATGCCTGTAGCTC	Shingfield et al. 2012
B. hungatei	BhuR	TCACCCTCGCGGGAT	Shingfield et al. 2012