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Table 2 LC–ESI–MS identification of surfactin and iturin type of homologues produced by B. velezensis SK, and comparison of their Molecular weight and peptide sequence reported in literature (Dhanarajan et al. 2016; Goswami and Deka 2019; Souza et al. 2018; De Faria et al. 2011; Dang et al. 2019; Liu et al. 2020; Chen et al. 2020)

From: Purification and characterization of antibacterial surfactin isoforms produced by Bacillus velezensis SK

RT (min) Observed MW
[M + H]+ (m/z)
Average MW reported Identified lipopeptide Relative % of lipopeptide family Amino acid sequence (Obtained from literature)
HP-20 Extract
Peak first
45.89 1043.58 1042.7 C14-Iturin 11.37 Cyclic (FA-N-Y-N-Q-P-N-S)
49.97 1057.59 1057.6 C15-Iturin 5.00 Cyclic (FA-N-Y-N-Q-P-N-S)
55.33 1071.61 1071.7 C16-Iturin 6.86 Cyclic (FA-N-Y-N-Q-P-N-S)
Peak second
63.52 994.67 994.21 C12-Surfactin 17.49 Cyclic (FA-E-L/I-L/I-V- L/I- D-L)
  1008.69 1008.87 C13-Surfactin 27.73 Cyclic (FA-E-L/I-L/I-V- L/I -D-L)
  1022.70 1023.18 C14-Surfactin 19.11 Cyclic (FA-E-L/I-L/I-V-V-D-L)
  1036.72 1036.78 C15 Surfactin 12.40 Cyclic (FA-E-L/I-L/I-V- L/I -D-L)
Fraction 2 (40% Acetone pH 4)
Peak first
45.88 1043.58 1042.7 C14-Iturin 0.71 Cyclic (FA-N-Y-N-Q-P-N-S)
50.48 1057.59 1057.6 C15-Iturin 0.20 Cyclic (FA-N-Y-N-Q-P-N-S)
Peak second
62.42 1036.72   C15-Surfactin 44.34 Cyclic (FA-E-L/I-L/I-V-D -L/I-L)
63.54 994.67 994.21 C12-Surfactin 54.74 Cyclic (FA-E-L/I-L/I-V-D-L/I-L)
  1008.69 1008.87 C13-Surfactin Cyclic (FA-E-L/I-L/I-V-D-L/I-L)
  1022.70 1023.18 C14-Surfactin Cyclic (FA-E-L/I-L/I-V-D-L/I-L)
  1036.72 1036.78 C15-Surfactin Cyclic (FA-E-L/I-L/I-V-D-L/I-L)
  1050.73 1050.92 C16-Surfactin Cyclic (FA-E-L/I-L/I-V-D-L/I-L)
Fraction 5 (80% Acetone pH 8)
62.20 994.67 994.21 C12-Surfactin 24.17 Cyclic (FA-E-L/I-L/I-V-D-L/I-L)
63.38 1022.70 1023.18 C14-Surfactin 32.18 Cyclic (FA-E-L/I-L/I-V-D-L/I-L)
  1036.72 1036.78 C15-Surfactin 43.64 Cyclic (FA-E-L/I-L/I-V-D-L/I-L)
  1. FA; Fatty acid, N: Asparagine; Y: Tyrosine; Q: Glutamine; P: Proline; S: Serine; E: Glutamic acid; L: Leucine; I: Isoleucine; V: Valine; D: Aspartic acid