Fig. 5

Thin layer chromatography (TLC) of partial purified lipopeptides from B. velezensis SK using a Solvent system-I and visualized with 0.2% ninhydrin, Lane 1, 2, 3, and 4 correspond to lipopeptide extracted with chloroform, lipopeptides produced in MBM, acid precipitated and methanol extracted lipopeptides and Diaion HP-20 lipopeptides respectively. b Solvent System-II and visualized by iodine vapour, TLC analysis of lipopeptide extracted by HP-20 lane 1 (MBM) and lane 2 (NB). c TLC plates of Diaion HP-20 extracted lipopeptide from NB visualized by UV 254 nm lane1, TSA lane 2, and Chloroform extracted lipopeptides lane 3 using solvent system-II. d In-situ antimicrobial activity of separated lipopeptides against B. cereus NCIM 2703 by using autobiography technique, and e Antimicrobial activity of TLC separated (upper spot) and eluted spots (disk 1) against B. cereus NCIM 2703 by paper disk assay. f Antimicrobial activity of TLC eluted (spot 1) bioactive spot by agar well diffusion assay. g TLC analysis of acid hydrolyzed purified lipopeptide fraction (Lane 2) and intact lipopeptide (Lane 1) as compared to amino acids Glutamate, Leucine, Aspartate and isoleucine lane 3, 4, 5, and 6 respectively after visualization by ninhydrin using solvent system-I