Fig. 4
The roles of tolA, tolB, and tolR in cell growth, motility, and virulence of S. Choleraesuis. a Growth curves of C78-3, ΔtolA, ΔtolB, and ΔtolR using OD600 measurements, and the bacterial suspensions were serially diluted and plated to determine CFU numbers per milliliter. b Motility was evaluated by measuring the growth halo of C78-3, ΔtolA, ΔtolB, and ΔtolR. Each assay was completed in 3 independently conducted biology duplicates. *P < 0.05; **P < 0.01; ***P < 0.001. c LD50 evaluation of C78-3, ΔtolA, ΔtolB, and ΔtolR with a murine model. The infected mice were supervised every day for 30 days