Fig. 3

Resistance to sodium deoxycholate and vancomycin of C78-3 and three mutants. a Survival of C78-3, ΔtolA, ΔtolB, and ΔtolR in 0.5% sodium deoxycholate. Bacterial strains were cultivated in LB to an OD₆₀₀ of 0.9. Then, the cells were resuspended in 0.5% sodium deoxycholate or PBS at 37 °C for 2 h. The survival rate was computed as (CFU in deoxycholic acid/CFU in PBS) × 100%. b Resistance to vancomycin of C78-3, ΔtolA, ΔtolB, and ΔtolR strains. The vancomycin disks contained 30 µg of the antibiotic. Each assay was completed in 3 independently conducted biology duplicates. *P < 0.05; **P < 0.01; ***P < 0.001