Fig. 2
From: Spontaneous quorum-sensing hierarchy reprogramming in Pseudomonas aeruginosa laboratory strain PAO1
Inactivating MexT responsible for the active Rhl QS phenotypes in PAO1-z. A Skim milk plate assay of indicated strains. The same amounts of bacteria were spotted onto the skim milk plate and photographed after 18 h incubation. B Detection of fluorescence of PrhlA-GFP reporter in indicated strains. The expression level of GFP in the tested strain was measured by the microplate reader and reported as relative fluorescence units (RFU). PAO1-u-LasR, PAO1-u strain with deleting the full-length of the lasR gene; PAO1-z + MexT, PAO1-z strain was complemented with a construct expressing the mexT gene from the PAO1-u strain; PAO1-u-LasR-MexT, PAO1-u strain with deleting both of the lasR and mexT genes