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Fig. 1 | AMB Express

Fig. 1

From: Spontaneous quorum-sensing hierarchy reprogramming in Pseudomonas aeruginosa laboratory strain PAO1

Fig. 1

QS activity profiles in P. aeruginosa laboratory strains. A Protease activity is shown in skim milk assay of three P. aeruginosa laboratory strains. An equal amount of bacteria were spotted on to the skim milk plate and photographed after 18 h of incubation. B The LasR-responsive activity in PAO1-u strain, PAO1-z strain and PAO1-u-LasR mutant. LasR-responsive activity was reflected by the fluorescence level of the PlasI-GFP reporter. The expression level of GFP in the tested strain was measured by the microplate reader and reported as relative fluorescence units (RFU). C Illustration of 3-bp insertion in the ligand-binding domain of the lasR gene in the PAO1-z strain. D Protease activity of PAO1-z strain and PAO1-z-LasR3 mutant in skim milk plate. PAO1-z-LasR3 mutant, PAO1-z strain with deleting the lasR3 variant

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