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Table 3 The effect of plant growth regulators on total phenolic (TPC), Total flavonoid (TFC), and anthocyanin (AC) content in the callus cultures of M. azedarach L.

From: Sterilization protocols and the effect of plant growth regulators on callus induction and secondary metabolites production in in vitro cultures Melia azedarach L.

Concentration of PGRs (mg/L) TPC (mg GAE/g FW) TFC (mg QE/g FW) AC (μm/g FW)
1 NAA  + 1 KIN 0.010 ± 0.005fg 82.805 ± 4.545b−g 1.650 ± 0.170bcd
1 NAA  + 1 BAP 0.021 ± 0.006d−h 80.402 ± 11.385b−g 1.508 ± 0.302bcd
1 2,4-D  + 1 KIN 0.027 ± 0.009d−g 67.522 ± 4.774efg 1.647 ± 0.042bcd
1 2,4-D  + 1 BAP 0.030 ± 0.006d−g 68.868 ± 5.132d−g 1.111 ± 0.208cd
3 NAA  + 1 KIN 0.026 ± 0.007d−g 66.465 ± 10.599fg 1.084 ± 0.116cd
3 NAA  + 1 BAP 0.051 ± 0.002cd 102.509 ± 13.155b−e 1.623 ± 0.563bcd
3 NAA  + 3 KIN 0.066 ± 0.011bc 101.644 ± 17.790b−g 1.946 ± 0.481abc
3 NAA  + 3 BAP 0.022 ± 0.010d−g 101.836 ± 14.609b−e 0.152 ± 0.077e
3 2,4-D  + 1 KIN 0.094 ± 0.007ab 107.507 ± 9.145bc 2.458 ± 0.281ab
3 2,4-D  + 1 BAP 0.043 ± 0.002c−f 100.202 ± 2.888b−f 2.707 ± 0.386a
3 2,4-D  + 3 KIN 0.090 ± 0.001ab 109.237 ± 6.528b 2.667 ± 0.545a
3 2,4-D  + 3 BAP 0.109 ± 0.007a 193.051 ± 16.732a 2.455 ± 0.293ab
5 NAA  + 1 KIN 0.013 ± 0.007efg 58.583 ± 13.115g 1.525 ± 0.417bcd
5 NAA  + 1 BAP 0.012 ± 0.006fg 68.291 ± 9.497d−g 1.6061 ± 0.102bcd
5 NAA  + 5 KIN 0.001 ± 0.014g 73.385 ± 13.732c−g 1.448 ± 0.288cd
5 NAA  + 5 BAP 0.017 ± 0.012efg 69.829 ± 10.656d−g 1.216 ± 0.218cd
5 2,4-D  + 1 KIN 0.046 ± 0.009cde 100.875 ± 2.217b−f 1.865 ± 0.084abc
5 2,4-D  + 1 BAP 0.041 ± 0.016c−f 103.278 ± 0.254bcd 1.872 ± 0.148abc
5 2,4-D  + 5 KIN 0.022 ± 0.019d−g 64.639 ± 11.731g 0.673 ± 0.139de
5 2,4-D  + 5 BAP 0.017 ± 0.008efg 70.406 ± 2.141d−g 0.741 ± 0.255de
  1. In each column, different letters indicate a significant difference (P ≤ 5%) according to Duncan’s Multiple-Range Tests (DMRTs)