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Table 1 The effect of different disinfection methods on the percentage of microbial contamination and in vitro response of M. azedarach L. leaf explants

From: Sterilization protocols and the effect of plant growth regulators on callus induction and secondary metabolites production in in vitro cultures Melia azedarach L.

Methods of disinfection Bacterial contamination (%) Fungal contamination (%) Clean explant (%) Viability (%) Browning (%) Callus induction (%) weight of callus (mg/explant)
Control 15.97 ± 3.10d 29.49 ± 4.08c 54.5 ± 4.8d 1.00 ± 0.00a 0.00 ± 0.00a 26.78 ± 5.58b 873.733 ± 34.418ab
A1 8.74 ± 1.56c 1.08 ± 0.45a 90.2 ± 1.6bc 1.00 ± 0.00a 0.00 ± 0.00a 5.91 ± 1.59c 484.568 ± 26.896bc
A2 5.57 ± 1.21bc 7.45 ± 1.47b 87.0 ± 2.4c 99.65 ± 0.35a 0.35 ± 0.35a 38.56 ± 5.49a 1191.294 ± 37.148a
A3 3.39 ± 0.94ab 0.00 ± 0.00a 96.6 ± 0.9ab 86.45 ± 3.29b 14.20 ± 3.25b 1.52 ± 0.91c 158.921 ± 96.003c
A4 0.40 ± 0.40a 0.00 ± 0.00a 99.6 ± 0.4a 79.25 ± 3.58c 21.14 ± 3.67c 9.41 ± 2.37c 554.056 ± 89.829bc
A5 0.86 ± 0.60a 0.69 ± 0.48a 98.5 ± 0.8a 99.31 ± 0.48a 0.79 ± 0.55a 10.77 ± 2.82c 282.111 ± 100.367c
A6 1.06 ± 0.60a 1.32 ± 0.63a 97.6 ± 0.8a 77.87 ± 3.94c 22.72 ± 4.01c 3.91 ± 1.43c 130.382 ± 60.386c
A7 0.37 ± 0.37a 0.00 ± 0.00a 99.6 ± 0.4a 54.48 ± 4.64d 45.52 ± 4.64d 9.44 ± 3.76c 108.147 ± 47.128c
  1. In each column, different letters indicate a significant difference (P ≤ 5%) according to Duncan’s Multiple-Range Tests (DMRTs)