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Table 1 The effect of different disinfection methods on the percentage of microbial contamination and in vitro response of M. azedarach L. leaf explants

From: Sterilization protocols and the effect of plant growth regulators on callus induction and secondary metabolites production in in vitro cultures Melia azedarach L.

Methods of disinfection

Bacterial contamination (%)

Fungal contamination (%)

Clean explant (%)

Viability (%)

Browning (%)

Callus induction (%)

weight of callus (mg/explant)

Control

15.97 ± 3.10d

29.49 ± 4.08c

54.5 ± 4.8d

1.00 ± 0.00a

0.00 ± 0.00a

26.78 ± 5.58b

873.733 ± 34.418ab

A1

8.74 ± 1.56c

1.08 ± 0.45a

90.2 ± 1.6bc

1.00 ± 0.00a

0.00 ± 0.00a

5.91 ± 1.59c

484.568 ± 26.896bc

A2

5.57 ± 1.21bc

7.45 ± 1.47b

87.0 ± 2.4c

99.65 ± 0.35a

0.35 ± 0.35a

38.56 ± 5.49a

1191.294 ± 37.148a

A3

3.39 ± 0.94ab

0.00 ± 0.00a

96.6 ± 0.9ab

86.45 ± 3.29b

14.20 ± 3.25b

1.52 ± 0.91c

158.921 ± 96.003c

A4

0.40 ± 0.40a

0.00 ± 0.00a

99.6 ± 0.4a

79.25 ± 3.58c

21.14 ± 3.67c

9.41 ± 2.37c

554.056 ± 89.829bc

A5

0.86 ± 0.60a

0.69 ± 0.48a

98.5 ± 0.8a

99.31 ± 0.48a

0.79 ± 0.55a

10.77 ± 2.82c

282.111 ± 100.367c

A6

1.06 ± 0.60a

1.32 ± 0.63a

97.6 ± 0.8a

77.87 ± 3.94c

22.72 ± 4.01c

3.91 ± 1.43c

130.382 ± 60.386c

A7

0.37 ± 0.37a

0.00 ± 0.00a

99.6 ± 0.4a

54.48 ± 4.64d

45.52 ± 4.64d

9.44 ± 3.76c

108.147 ± 47.128c

  1. In each column, different letters indicate a significant difference (P ≤ 5%) according to Duncan’s Multiple-Range Tests (DMRTs)