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AMB Express
Fig. 1 | AMB Express

Fig. 1

From: φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3

Fig. 1

SDS-PAGE of TFP-Gp17. A Samples from all purification steps by nickel-affinity chromatography. Lanes: 1, supernatant from bacterial lysate; 2, flow through 1 (FT1) containing the unbound proteins; 3, elution fraction 1 (elution with buffer A supplemented with 250 mM imidazole); 4, elution fraction after desalting with ammonium sulfate; 5,7, FT2, containing the unbound proteins after cleavage with the TEV protease; 6, elution fraction 2. B TFP-Gp17 before SEC chromatography is shown in the lane 6. C Protein fractions after SEC chromatography; TFP-Gp17 in 4 lane is indicated by the red arrow. D The H/MTFP-Gp17 protein after SEC chromatography is indicated in lane by the red arrow

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