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AMB Express
Fig. 2 | AMB Express

Fig. 2

From: Thermococcus sp. KS-1 PPIase as a fusion partner improving soluble production of aromatic amino acid decarboxylase

Fig. 2

Overproduction of AADC, PPIase KS−1-AADC and NusA-AADC. A Cell-free extract from each E.coli overexpressing strain was analyzed by SDS-PAGE. Prestained Protein Marker, Broad Range (New England Biolabs) was used as a molecular weight marker. Soluble and insoluble overexpressed target proteins were indicated by filled and open arrows respectively. B AADC activity in cell-free extracts of the respective overexpressing strains. The values are indicated as mean ± standard deviation. Statistical significance (*p < 0.05; **p < 0.01) was determined with Tukey’s multiple comparisons test for four measurements

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