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Table 1 Kinetic and respirometric parameters of A. vinelandii cultured in shake flasks in different culture media and conditions

From: Growth, respiratory activity and chlorpyrifos biodegradation in cultures of Azotobacter vinelandii ATCC 12837

Culture medium Specific growth rate (μ) Duplication time (h) Final alginate (gL−1) Protein (mg mL−1) CFUs (mL−1) OTRmax (mmol L −1 h−1) qO2 max (mmol /mg mL−1 h−1)
NBRC 0.03 9.45 0.49 ± 0.03 0.14 ± 0.01 1.25 × 109 2.65 18.92
NBRCm 0.08 6.30 5.17 ± 0.56 1.12 ± 0.06 9.16 × 109 5.87 5.24
BS 0.12 5.55 0.97 ± 0.12 1.79 ± 0.02 1.4 × 1010 5.52 3.08
BS medium with different filling volume (mL) Specific growth rate (μ) Duplication time (h) Biomass (g L−1) Protein (mg mL−1) CFUs mL−1 OTRmax (mmol L−1 h−1) qO2max (mmol /mg mL−1 h−1)
50 0.12 5.77 9.77 ± 0.65 2.78 ± 0.26 1 × 1010 5.5 1.97
20 0.08 6.30 4.67 ± 0.18 1.04 ± 0.28 8.2 × 108 11.45 11
10 0.11 5.80 5.90 ± 0.95 2.12 ± 0.05 9.4 × 109 22 10.37
Culture medium Specific growth rate (μ) Duplication time (h) Biomass (g L−1) Protein (mg mL−1) CFUs mL−1 OTRmax (mmol L−1 h−1) qO2 max (mmol /mg mL−1 h−1)
BS 0.26 2.77 8.9 ± 0.1 1.82 ± 0.21 9 × 109 5.88 2.66
BS + CP 0.26 2.83 10.4 ± 0.05 3.32 ± 0.18 1 × 1010 7.9 2.37
BS2 0.14 5.05 2.27 ± 0.2 0.59 ± 0.06 2.1 × 107 5.38 9.11
BS2 + CP 0.19 4.45 2.93 ± 0.02 0.63 ± 0.04 2.51 × 107 6.9 10.9
  1. Experiments were carried out in triplicate and the results presented are the averages and standard deviation of independent runs