Growth, respiratory activity and chlorpyrifos biodegradation in cultures of Azotobacter vinelandii ATCC 12837

AMB Express

Table 1 Kinetic and respirometric parameters of A. vinelandii cultured in shake flasks in different culture media and conditions

Culture medium	Specific growth rate (μ)	Duplication time (h)	Final alginate (gL⁻¹)	Protein (mg mL⁻¹)	CFUs (mL⁻¹)	OTR_max (mmol L ⁻¹ h⁻¹)	qO_{2 max} (mmol /mg mL⁻¹ h⁻¹)
NBRC	0.03	9.45	0.49 ± 0.03	0.14 ± 0.01	1.25 × 10⁹	2.65	18.92
NBRCm	0.08	6.30	5.17 ± 0.56	1.12 ± 0.06	9.16 × 10⁹	5.87	5.24
BS	0.12	5.55	0.97 ± 0.12	1.79 ± 0.02	1.4 × 10¹⁰	5.52	3.08

BS medium with different filling volume (mL)	Specific growth rate (μ)	Duplication time (h)	Biomass (g L⁻¹)	Protein (mg mL⁻¹)	CFUs mL⁻¹	OTR_max (mmol L⁻¹ h⁻¹)	qO2_max (mmol /mg mL⁻¹ h⁻¹)
50	0.12	5.77	9.77 ± 0.65	2.78 ± 0.26	1 × 10¹⁰	5.5	1.97
20	0.08	6.30	4.67 ± 0.18	1.04 ± 0.28	8.2 × 10⁸	11.45	11
10	0.11	5.80	5.90 ± 0.95	2.12 ± 0.05	9.4 × 10⁹	22	10.37

Culture medium	Specific growth rate (μ)	Duplication time (h)	Biomass (g L⁻¹)	Protein (mg mL⁻¹)	CFUs mL⁻¹	OTR_max (mmol L⁻¹ h⁻¹)	qO_{2 max} (mmol /mg mL⁻¹ h⁻¹)
BS	0.26	2.77	8.9 ± 0.1	1.82 ± 0.21	9 × 10⁹	5.88	2.66
BS + CP	0.26	2.83	10.4 ± 0.05	3.32 ± 0.18	1 × 10¹⁰	7.9	2.37
BS2	0.14	5.05	2.27 ± 0.2	0.59 ± 0.06	2.1 × 10⁷	5.38	9.11
BS2 + CP	0.19	4.45	2.93 ± 0.02	0.63 ± 0.04	2.51 × 10⁷	6.9	10.9

Experiments were carried out in triplicate and the results presented are the averages and standard deviation of independent runs