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Table 1 List of strains and plasmids used in this study

From: Modular control of multiple pathways of Corynebacterium glutamicum for 5-aminolevulinic acid production

Strain and plasmids Genotype or description References
Strain
 C. glutamicum F1 The parental strain C.glutamicum F343 Provided kindly by Dr. Pu Zheng, School of biotechnology, Jiangnan university (Zheng et al 2012)
 C. glutamicum F2 C.glutamicum F343 ∆gdhA This study
 C. glutamicum F3 C.glutamicum F343 ∆gdhAaceA This study
 C. glutamicum F1-P C. glutamicum harboring pXMJ19 This study
 C. glutamicum F1-A Glutamicum harboring pXMJ19-hemAC4 This study
 C. glutamicum F2-A C. glutamicumgdhA harboring pXMJ19-hemAC4 This study
 C. glutamicum F3-A C.glutamicum F343 ∆gdhAaceA harboring pXMJ19-hemAC4 This study
 E. coli DH5α Wild-type strain; subcloning host Lab stock
Plasmids
 pK18mobsacB Mobilizable E.coli cloning vector, Kmr Lab stock
 pK18mobsacB-∆gdhA Integrative transformation vector for deletion of the gdhA gene This study
 pET28a( +)-hemA pET28a ( +) carrying hemA from R. capsulatus Lab stock
 pXMJ19-hemAC4 pXMJ19 containing hemAC4, for overexpression This study
 pK18mobsacB-∆aceA Integrative transformation vector for deletion of the aceA gene This study