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Table 2 Concentration of ADP ribosylated NBAG formed post exposure to irradiated exotoxin A protein extract and percent reduction compared to the un-irradiated exotoxin A

From: ADP-ribosyl transferase activity and gamma radiation cytotoxicity of Pseudomonas aeruginosa exotoxin A

Radiation dosage Retention time at 301 nm Peak area ʎ max Conc. of unknown (ADP-ribosylated products) Percent of ADP-ribosylated products formed Percent reduction in the amount of ADP-ribosylated product formed
P. aeruginosa 16 (24 Gy) NBAG peak 9.38 11,632,146 314/228 0.01  
P. aeruginosa 16 (24 Gy) new peaks 10.6 1300 223/291/327 1.118 × 10–6 0.73 0.98
12.14 11,518 225/340 9.902 × 10–6
12.24 17,167 225/327 1.476 × 10–6
13.17 2415 225 2.076 × 10–6
13.54 8416 226 7.235 × 10–6
P. aeruginosa 22 (24 Gy) NBAG peak 9.34 10,739,513 325/225 0.01  
P. aeruginosa 22 (24 Gy) new peaks 10.56 2654 222/291/269/327 2.5 × 10–6 0.71 0.14
12.08 21,741 225/340 2.5 × 10–6
12.25 16,007 226/331 1.49 × 10–5
12.94 1606 226/331 1.5 × 10–6
13.19 2474 227/327 2.3 × 10–6
13.56 5403 227 5 × 10–6
13.72 4247 228/327 4 × 10–6
  1. Conc. of ADP ribosylated NBAG = (area unknown/area known) × conc. of NBAG; Percent ADP ribosylated NBAG formed = (total conc. of ADP ribosylated NBAG/conc. of NBAG) × 100; Percent reduction compared to un-irradiated strain = percent of ADP ribosylated NBAG formed for un-irradiated – percent of ADP ribosylated NBAG formed for irradiated