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Table 2 Concentration of ADP ribosylated NBAG formed post exposure to irradiated exotoxin A protein extract and percent reduction compared to the un-irradiated exotoxin A

From: ADP-ribosyl transferase activity and gamma radiation cytotoxicity of Pseudomonas aeruginosa exotoxin A

Radiation dosage

Retention time at 301 nm

Peak area

ʎ max

Conc. of unknown (ADP-ribosylated products)

Percent of ADP-ribosylated products formed

Percent reduction in the amount of ADP-ribosylated product formed

P. aeruginosa 16 (24 Gy) NBAG peak

9.38

11,632,146

314/228

0.01

 

P. aeruginosa 16 (24 Gy) new peaks

10.6

1300

223/291/327

1.118 × 10–6

0.73

0.98

12.14

11,518

225/340

9.902 × 10–6

12.24

17,167

225/327

1.476 × 10–6

13.17

2415

225

2.076 × 10–6

13.54

8416

226

7.235 × 10–6

P. aeruginosa 22 (24 Gy) NBAG peak

9.34

10,739,513

325/225

0.01

 

P. aeruginosa 22 (24 Gy) new peaks

10.56

2654

222/291/269/327

2.5 × 10–6

0.71

0.14

12.08

21,741

225/340

2.5 × 10–6

12.25

16,007

226/331

1.49 × 10–5

12.94

1606

226/331

1.5 × 10–6

13.19

2474

227/327

2.3 × 10–6

13.56

5403

227

5 × 10–6

13.72

4247

228/327

4 × 10–6

  1. Conc. of ADP ribosylated NBAG = (area unknown/area known) × conc. of NBAG; Percent ADP ribosylated NBAG formed = (total conc. of ADP ribosylated NBAG/conc. of NBAG) × 100; Percent reduction compared to un-irradiated strain = percent of ADP ribosylated NBAG formed for un-irradiated – percent of ADP ribosylated NBAG formed for irradiated
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