Skip to main content
AMB Express

Table 1 Concentration of ADP ribosylated NBAG and percent ADP ribosylated NBAG formed post exposure to exotoxin A protein extract at 301 nm

From: ADP-ribosyl transferase activity and gamma radiation cytotoxicity of Pseudomonas aeruginosa exotoxin A

P. aeruginosa strains (Code number of tested isolate) Retention time at 301 nm ʎ max of the minor peaks Peak area ADP-ribosylated NBAG formed in M Percent ADP ribosylated NBAG formed
PA 1 NBAG peak 9.33 329/227 18,583,078 0.01  
PA 1 new peaks 12.16 228/340 45,548 1.46 × 10–5 0.31
12.29 229/327 53,355 1.71 × 10–5
PA 5 NBAG peak 9.47 304/325/230 30,528,050 0.01  
PA 5 new peaks 12.29 225/327 35,634 1.16 × 10–5 0.23
13.58 226/268 33,613 1.101 × 10–5
PA 16 NBAG peak 9.32 331/228 21,872,503 0.01  
PA 16 new peaks 11.44 225/327 77,762 3.55 × 10–5 1.71
11.6 225/327 60,768 2.78 × 10–5
12.05 226/339 123,413 5.64 × 10–5
12.31 226/327 63,771 2.91 × 10–5
12.46 226/327 49,347 2.26 × 10–5
11.44 225/327 77,762 3.55 × 10–5
11.6 225/327 60,768 2.77 × 10–5
12.05 226/339 123,413 5.64 × 10–5
PA 22 NBAG peak 9.32 332 18,955,192 0.01  
PA 22 new peaks 11.17 204/266/329/367/288 3050 1.61 × 10–6 0.85
12.08 205/340 62,448 3.29 × 10–5
12.23 206/329 22,983 1.212 × 10–5
12.4 206/265/329/368 3215 1.7 × 10–6
12.92 208/265/329/369 2151 1.13 × 10–6
13.17 208/323/369 6124 3.23 × 10–6
PA 35 NBAG peak 9.43 334/231 31,719,819 0.01  
PA 35 new peaks 12.16 224/339 44,585 1.41 × 10–5 0.42
12.29 225/327 48,434 1.53 × 10–5
13.58 225/268 42,470 1.34 × 10–5
PA 39 NBAG peak 9.33 329/227 18,583,078 0.01  
PA 39 new peaks 12.08 226/340 62,029 3.34 × 10–5 0.33
  1. Conc. = concentration; Concentration of ADP ribosylated NBAG = (area unknown/area known) × concentration of known (NBAG); Percent reduction in NBAG conc. = (total concentration of ADP ribosylated NBAG/concentration of NBAG) × 100
Back to article page