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AMB Express

Table 1 Concentration of ADP ribosylated NBAG and percent ADP ribosylated NBAG formed post exposure to exotoxin A protein extract at 301 nm

From: ADP-ribosyl transferase activity and gamma radiation cytotoxicity of Pseudomonas aeruginosa exotoxin A

P. aeruginosa strains (Code number of tested isolate)

Retention time at 301 nm

ʎ max of the minor peaks

Peak area

ADP-ribosylated NBAG formed in M

Percent ADP ribosylated NBAG formed

PA 1 NBAG peak

9.33

329/227

18,583,078

0.01

 

PA 1 new peaks

12.16

228/340

45,548

1.46 × 10–5

0.31

12.29

229/327

53,355

1.71 × 10–5

PA 5 NBAG peak

9.47

304/325/230

30,528,050

0.01

 

PA 5 new peaks

12.29

225/327

35,634

1.16 × 10–5

0.23

13.58

226/268

33,613

1.101 × 10–5

PA 16 NBAG peak

9.32

331/228

21,872,503

0.01

 

PA 16 new peaks

11.44

225/327

77,762

3.55 × 10–5

1.71

11.6

225/327

60,768

2.78 × 10–5

12.05

226/339

123,413

5.64 × 10–5

12.31

226/327

63,771

2.91 × 10–5

12.46

226/327

49,347

2.26 × 10–5

11.44

225/327

77,762

3.55 × 10–5

11.6

225/327

60,768

2.77 × 10–5

12.05

226/339

123,413

5.64 × 10–5

PA 22 NBAG peak

9.32

332

18,955,192

0.01

 

PA 22 new peaks

11.17

204/266/329/367/288

3050

1.61 × 10–6

0.85

12.08

205/340

62,448

3.29 × 10–5

12.23

206/329

22,983

1.212 × 10–5

12.4

206/265/329/368

3215

1.7 × 10–6

12.92

208/265/329/369

2151

1.13 × 10–6

13.17

208/323/369

6124

3.23 × 10–6

PA 35 NBAG peak

9.43

334/231

31,719,819

0.01

 

PA 35 new peaks

12.16

224/339

44,585

1.41 × 10–5

0.42

12.29

225/327

48,434

1.53 × 10–5

13.58

225/268

42,470

1.34 × 10–5

PA 39 NBAG peak

9.33

329/227

18,583,078

0.01

 

PA 39 new peaks

12.08

226/340

62,029

3.34 × 10–5

0.33

  1. Conc. = concentration; Concentration of ADP ribosylated NBAG = (area unknown/area known) × concentration of known (NBAG); Percent reduction in NBAG conc. = (total concentration of ADP ribosylated NBAG/concentration of NBAG) × 100
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