Improvement of putrescine production through the arginine decarboxylase pathway in Escherichia coli K-12

AMB Express

Table 2 Plasmids used in this study

Strains	Genotype	Source or reference
pCP20	pSC101 replicon (Ts) bla⁺ cat⁺	Datsenko and Wanner 2000
	Flp(γRp) d857
pFS29	pQE-80L except T5p lacO_lacO_speC⁺	This study
pKD13	oriRγ bla⁺ FRT-kan^R-FRT	Datsenko and Wanner 2000
pKN11	pQE-80L except T5p lacO_lacO	This study
	_speA_speB_argA^{ATG Y19C}
pKN18	pQE-80L except T5p lacO_lacO	This study
	_speA_speB_argA^{ATG Y19C} T5p lacO_
	lacO_speC
pKT199	pQE-80L except	This study
	lacI^q1p_lacO_speA_speB_argA^{GTG Y19C}
	lacI
pKT200	pQE-80L except	This study
	lacI^q1p_lacO_speA_speB_argA^{ATG Y19}
	lacI
pKT201	pQE-80L except	This study
	lacI^q1p_lacO_speA_speB_argA^{GTG Y19}
	lacI
pQE-80L	ColE1 replicon bla⁺ lacI^q T5p	Qiagen
	lacO_lacO-(His)6
pSH1733	pQE-80L except argA^{ATG Y19C}	This study
pSH1734	pQE-80L except T5p lacO_lacO_speC⁺	This study
	_ argA^{ATG Y19C}
pSH1807	pQE-80L except	This study
	lacI^qp_lacO_speA_speB_argA^{ATG Y19C}
	lacI
pSH1820	pQE-80L except	This study
	lacI^q1p_lacO_speA_speB_argA^{ATG Y19C}
	lacI
pSH1823	pQE-80L except	This study
	lacIp_lacO_speA_speB_argA^{ATG Y19C}
	lacI