Fig. 7
Effects of culture media on putrescine production by strain KT152. A Bacterial cell growth and B putrescine concentration in the supernatant of the strain KT152, whose putrescine utilization pathway (ΔpuuAP ΔpatA) was disrupted. Open triangles represent the strain KT152 grown in the LB medium supplemented with 100 μg/mL of ampicillin; closed circles represent the strain KT152 grown in terrific broth supplemented with 100 μg/mL of ampicillin. When the OD600 reached 0.4, 0.03 mM IPTG was added. Data shown are averages ± standard deviations, and culture experiments were performed in triplicate