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Table 1 List of engineered ArsR and metal(loid)s selectivity upon host E. coli strains

From: Derivation of pb(II)-sensing Escherichia coli cell-based biosensors from arsenic responsive genetic systems

No Name Amino acid sequence (no. 30–40) Cysteine location Order of responding strength References
E. coli-arsR E. coli-arsR/copA E. coli-arsR/zntA
1 WT ELCVCDLCTA Cys-32,34,37 As(9.8) As(8.46) As (7.38)>Pb (1.85) Kim et al. (2020)
2 W4 ELCVCDLSTC Cys-32,34,39 As(5.7)>>Cd(1.95) As(3.64) Pb(3.03)>As(2.31)> Cd(1.73) Lee et al. (2020)
3 W6 ELCVCDCSTA Cys-32,34,36 As(5.08) As(4.23) Pb(4.05)>Cd(2.56)>As(1.79) Lee et al. (2020)
4 YJ6 CLCVCDLSTC Cys-30,32,34,39 As(3.34) As(1.58) Pb(2.31)>As(1.77)>Cd(1.53) This study
5 YJ20 ELCVCDLCTC Cys-32,34,37,39 As(4.54) As(2.43) As (2.71)>Pb (2.31)>Cd(1.3) This study
6 W2 ELCVCDLSTA Cys-32,34 As(11.34) As(10.46) As(9.98)>>Pb(2.06) Lee et al. (2020)
7 W3 ELCVCDLSCA Cys-32,34,38 As(18.40) As(8.87) As(9.13)>>Pb(1.67)>Cd(1.60) Lee et al. (2020)
8 YJ2 CLCVCDLSTA Cys-30,32,34 As(3.06) As(2.21) As (2.50)>Pb(1.57) This study
9 YJ1 CLSVCDLCTA Cys-30,34,37 This study
10 YJ4 ECSVCDLSTC Cys-31,34,39 This study
11 YJ5 CLSVCDLSTC Cys-30,34,39 This study
12 YJ11 ELCVSCLSTC Cys-32,35,39 This study
13 YJ14 ELCCSDLSCA Cys-32,33,38 This study
14 YJ15 ELCCSDLSTC Cys-32,33,39 This study
15 YJ19 ELCVSDLCTC Cys-32,37,39 This study
16 YJ21 ELSVCDLSTC Cys-34,39 This study
17 YJ3 CLCVCDLCTA Cys-30,32,34,37 As(4.26) As(2.16) As(2.40) This study
18 YJ7 CLSVCDCSTA Cys-30,34,36 This study
19 YJ8 CLCVCDCSTA Cys-30,32,34,36 This study
20 YJ12 ELCCCDLSCA Cys-32,33,34,38 As (1.50) Cd(1.60)>Pb(1.38) This study
21 YJ13 ELCCCDLSTC Cys-32,33,34,39 As (2.41) As(1.61) Pb(1.43)>As(1.28)>Cd(1.26) This study
22 YJ16 ELSCCDLSCA Cys-33,34,38 This study
23 YJ17 ELSCCDLSTC Cys-33,34,39 This study
24 YJ18 ELCCCDLSTA Cys-32,33,34 This study
  1. + Cysteines in amino acid sequences were indicated as bold letters and mutated amino acids were indicated with underlines
  2. Responding strengths toward heavy metal(loid) ions were indicated with induction coefficient values and the values under 1.25 were not shown
  3. * The data were obtained by repeating more than 3 times and the standard deviations for induction coefficient values toward each metal(loid) was less than 10%