Skip to main content
AMB Express

Table 1 List of engineered ArsR and metal(loid)s selectivity upon host E. coli strains

From: Derivation of pb(II)-sensing Escherichia coli cell-based biosensors from arsenic responsive genetic systems

No Name Amino acid sequence (no. 30–40) Cysteine location Order of responding strength References
E. coli-arsR E. coli-arsR/copA E. coli-arsR/zntA
1 WT ELCVCDLCTA Cys-32,34,37 As(9.8) As(8.46) As (7.38)>Pb (1.85) Kim et al. (2020)
2 W4 ELCVCDLSTC Cys-32,34,39 As(5.7)>>Cd(1.95) As(3.64) Pb(3.03)>As(2.31)> Cd(1.73) Lee et al. (2020)
3 W6 ELCVCDCSTA Cys-32,34,36 As(5.08) As(4.23) Pb(4.05)>Cd(2.56)>As(1.79) Lee et al. (2020)
4 YJ6 CLCVCDLSTC Cys-30,32,34,39 As(3.34) As(1.58) Pb(2.31)>As(1.77)>Cd(1.53) This study
5 YJ20 ELCVCDLCTC Cys-32,34,37,39 As(4.54) As(2.43) As (2.71)>Pb (2.31)>Cd(1.3) This study
6 W2 ELCVCDLSTA Cys-32,34 As(11.34) As(10.46) As(9.98)>>Pb(2.06) Lee et al. (2020)
7 W3 ELCVCDLSCA Cys-32,34,38 As(18.40) As(8.87) As(9.13)>>Pb(1.67)>Cd(1.60) Lee et al. (2020)
8 YJ2 CLCVCDLSTA Cys-30,32,34 As(3.06) As(2.21) As (2.50)>Pb(1.57) This study
9 YJ1 CLSVCDLCTA Cys-30,34,37 –‡ – – This study
10 YJ4 ECSVCDLSTC Cys-31,34,39 – – – This study
11 YJ5 CLSVCDLSTC Cys-30,34,39 – – – This study
12 YJ11 ELCVSCLSTC Cys-32,35,39 – – – This study
13 YJ14 ELCCSDLSCA Cys-32,33,38 – – – This study
14 YJ15 ELCCSDLSTC Cys-32,33,39 – – – This study
15 YJ19 ELCVSDLCTC Cys-32,37,39 – – – This study
16 YJ21 ELSVCDLSTC Cys-34,39 – – – This study
17 YJ3 CLCVCDLCTA Cys-30,32,34,37 As(4.26) As(2.16) As(2.40) This study
18 YJ7 CLSVCDCSTA Cys-30,34,36 – – – This study
19 YJ8 CLCVCDCSTA Cys-30,32,34,36 – – – This study
20 YJ12 ELCCCDLSCA Cys-32,33,34,38 As (1.50) – Cd(1.60)>Pb(1.38) This study
21 YJ13 ELCCCDLSTC Cys-32,33,34,39 As (2.41) As(1.61) Pb(1.43)>As(1.28)>Cd(1.26) This study
22 YJ16 ELSCCDLSCA Cys-33,34,38 – – – This study
23 YJ17 ELSCCDLSTC Cys-33,34,39 – – – This study
24 YJ18 ELCCCDLSTA Cys-32,33,34 – – – This study
  1. + Cysteines in amino acid sequences were indicated as bold letters and mutated amino acids were indicated with underlines
  2. ‡ Responding strengths toward heavy metal(loid) ions were indicated with induction coefficient values and the values under 1.25 were not shown
  3. * The data were obtained by repeating more than 3 times and the standard deviations for induction coefficient values toward each metal(loid) was less than 10%
Back to article page