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Table 1 List of engineered ArsR and metal(loid)s selectivity upon host E. coli strains

From: Derivation of pb(II)-sensing Escherichia coli cell-based biosensors from arsenic responsive genetic systems

No

Name

Amino acid sequence (no. 30–40)

Cysteine location

Order of responding strength

References

E. coli-arsR

E. coli-arsR/copA

E. coli-arsR/zntA

1

WT

ELCVCDLCTA

Cys-32,34,37

As(9.8)

As(8.46)

As (7.38)>Pb (1.85)

Kim et al. (2020)

2

W4

ELCVCDLSTC

Cys-32,34,39

As(5.7)>>Cd(1.95)

As(3.64)

Pb(3.03)>As(2.31)> Cd(1.73)

Lee et al. (2020)

3

W6

ELCVCDCSTA

Cys-32,34,36

As(5.08)

As(4.23)

Pb(4.05)>Cd(2.56)>As(1.79)

Lee et al. (2020)

4

YJ6

CLCVCDLSTC

Cys-30,32,34,39

As(3.34)

As(1.58)

Pb(2.31)>As(1.77)>Cd(1.53)

This study

5

YJ20

ELCVCDLCTC

Cys-32,34,37,39

As(4.54)

As(2.43)

As (2.71)>Pb (2.31)>Cd(1.3)

This study

6

W2

ELCVCDLSTA

Cys-32,34

As(11.34)

As(10.46)

As(9.98)>>Pb(2.06)

Lee et al. (2020)

7

W3

ELCVCDLSCA

Cys-32,34,38

As(18.40)

As(8.87)

As(9.13)>>Pb(1.67)>Cd(1.60)

Lee et al. (2020)

8

YJ2

CLCVCDLSTA

Cys-30,32,34

As(3.06)

As(2.21)

As (2.50)>Pb(1.57)

This study

9

YJ1

CLSVCDLCTA

Cys-30,34,37

This study

10

YJ4

ECSVCDLSTC

Cys-31,34,39

This study

11

YJ5

CLSVCDLSTC

Cys-30,34,39

This study

12

YJ11

ELCVSCLSTC

Cys-32,35,39

This study

13

YJ14

ELCCSDLSCA

Cys-32,33,38

This study

14

YJ15

ELCCSDLSTC

Cys-32,33,39

This study

15

YJ19

ELCVSDLCTC

Cys-32,37,39

This study

16

YJ21

ELSVCDLSTC

Cys-34,39

This study

17

YJ3

CLCVCDLCTA

Cys-30,32,34,37

As(4.26)

As(2.16)

As(2.40)

This study

18

YJ7

CLSVCDCSTA

Cys-30,34,36

This study

19

YJ8

CLCVCDCSTA

Cys-30,32,34,36

This study

20

YJ12

ELCCCDLSCA

Cys-32,33,34,38

As (1.50)

Cd(1.60)>Pb(1.38)

This study

21

YJ13

ELCCCDLSTC

Cys-32,33,34,39

As (2.41)

As(1.61)

Pb(1.43)>As(1.28)>Cd(1.26)

This study

22

YJ16

ELSCCDLSCA

Cys-33,34,38

This study

23

YJ17

ELSCCDLSTC

Cys-33,34,39

This study

24

YJ18

ELCCCDLSTA

Cys-32,33,34

This study

  1. + Cysteines in amino acid sequences were indicated as bold letters and mutated amino acids were indicated with underlines
  2. Responding strengths toward heavy metal(loid) ions were indicated with induction coefficient values and the values under 1.25 were not shown
  3. * The data were obtained by repeating more than 3 times and the standard deviations for induction coefficient values toward each metal(loid) was less than 10%