Table 1 List of engineered ArsR and metal(loid)s selectivity upon host E. coli strains
No | Name | Amino acid sequence (no. 30–40) | Cysteine location | Order of responding strength | References | ||
|---|---|---|---|---|---|---|---|
E. coli-arsR | E. coli-arsR/copA | E. coli-arsR/zntA | |||||
1 | WT | ELCVCDLCTA | Cys-32,34,37 | As(9.8) | As(8.46) | As (7.38)>Pb (1.85) | Kim et al. (2020) |
2 | W4 | ELCVCDLSTC | Cys-32,34,39 | As(5.7)>>Cd(1.95) | As(3.64) | Pb(3.03)>As(2.31)> Cd(1.73) | Lee et al. (2020) |
3 | W6 | ELCVCDCSTA | Cys-32,34,36 | As(5.08) | As(4.23) | Pb(4.05)>Cd(2.56)>As(1.79) | Lee et al. (2020) |
4 | YJ6 | CLCVCDLSTC | Cys-30,32,34,39 | As(3.34) | As(1.58) | Pb(2.31)>As(1.77)>Cd(1.53) | This study |
5 | YJ20 | ELCVCDLCTC | Cys-32,34,37,39 | As(4.54) | As(2.43) | As (2.71)>Pb (2.31)>Cd(1.3) | This study |
6 | W2 | ELCVCDLSTA | Cys-32,34 | As(11.34) | As(10.46) | As(9.98)>>Pb(2.06) | Lee et al. (2020) |
7 | W3 | ELCVCDLSCA | Cys-32,34,38 | As(18.40) | As(8.87) | As(9.13)>>Pb(1.67)>Cd(1.60) | Lee et al. (2020) |
8 | YJ2 | CLCVCDLSTA | Cys-30,32,34 | As(3.06) | As(2.21) | As (2.50)>Pb(1.57) | This study |
9 | YJ1 | CLSVCDLCTA | Cys-30,34,37 | –‡ | – | – | This study |
10 | YJ4 | ECSVCDLSTC | Cys-31,34,39 | – | – | – | This study |
11 | YJ5 | CLSVCDLSTC | Cys-30,34,39 | – | – | – | This study |
12 | YJ11 | ELCVSCLSTC | Cys-32,35,39 | – | – | – | This study |
13 | YJ14 | ELCCSDLSCA | Cys-32,33,38 | – | – | – | This study |
14 | YJ15 | ELCCSDLSTC | Cys-32,33,39 | – | – | – | This study |
15 | YJ19 | ELCVSDLCTC | Cys-32,37,39 | – | – | – | This study |
16 | YJ21 | ELSVCDLSTC | Cys-34,39 | – | – | – | This study |
17 | YJ3 | CLCVCDLCTA | Cys-30,32,34,37 | As(4.26) | As(2.16) | As(2.40) | This study |
18 | YJ7 | CLSVCDCSTA | Cys-30,34,36 | – | – | – | This study |
19 | YJ8 | CLCVCDCSTA | Cys-30,32,34,36 | – | – | – | This study |
20 | YJ12 | ELCCCDLSCA | Cys-32,33,34,38 | As (1.50) | – | Cd(1.60)>Pb(1.38) | This study |
21 | YJ13 | ELCCCDLSTC | Cys-32,33,34,39 | As (2.41) | As(1.61) | Pb(1.43)>As(1.28)>Cd(1.26) | This study |
22 | YJ16 | ELSCCDLSCA | Cys-33,34,38 | – | – | – | This study |
23 | YJ17 | ELSCCDLSTC | Cys-33,34,39 | – | – | – | This study |
24 | YJ18 | ELCCCDLSTA | Cys-32,33,34 | – | – | – | This study |