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Table 1 Optimization of low-cost substrate and culture conditions for lipopeptide production by Bacillus subtilis SNW3

From: Low-cost production and application of lipopeptide for bioremediation and plant growth by Bacillus subtilis SNW3

Factors Ranges
Temperature (°C) 15, 30, 37 and 50
pH 2, 4, 6, 8, 10, 12
Agitation speed (rpm) 0, 150, 250
Inoculum size (%, w/v) 0.5, 1, 1.5, 2, 2.5
Nitrogen source (0.1%, w/v) Urea, sodium nitrate, ammonium nitrate
Carbon source (conc. 2%, w/v) White beans powder, potato peels powder, molasses, waste frying oil, yeast extract
Carbon and nitrogen source ratio (W.B.P + W.F.O + U) w/v 11 + 0.5 + 0.1, 8 + 1 + 0.1, 6 + 1.5 + 0.1, 3 + 2 + 0.1