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Table 7 Comparison between cumulative mortality of Aedes aegypti larvae after treatment with Metarhizium robertsii ARSEF 2575

From: Metarhizium robertsii protease and conidia production, response to heat stress and virulence against Aedes aegypti larvae

Treat Stress SUPPL UNSUPPL Treat Stress SUPPL UNSUPPL Treat Stress SUPPL UNSUPPL
Mortality (%) Mortality (%) Mortality (%)
3 Days 5 Days 10 Days
Crude extract None 66.7 ± 8.8A 80.0 ± 5.8A Crude extract None 90.0 ± 5.8A 96.7 ± 3.3A Crude extract None 100 ± 0A 100.0 ± 0A
Crude extract Heat 60.0 ± 5.8A 50.0 ± 5.8A Crude extract Heat 76.0 ± 3.3A 83.3 ± 12.0A Crude extract Heat 93.3 ± 3.3A 96.7 ± 3.3A
Conidia None 60.0 ± 5.8A 66.7 ± 12.0A Conidia None 80.0 ± 10.0A 90.0 ± 5.8A Conidia None 100 ± 0A 96.7 ± 3.3A
Conidia Heat 53.3 ± 3.33A 66.7 ± 6.7A Conidia Heat 83.3 ± 6.7A 90.0 ± 5.8A Conidia Heat 100 ± 0A 96.7 ± 3.3A
Supernatant None 15.0 ± 5.0A 15.0 ± 5.0A Supernatant None 15.0 ± 5.0A 20.0 ± 0A Supernatant None 30.0 ± 10.0A 35.0 ± 5.0A
Supernatant Heat 70.0 ± 20.0B 15.0 ± 15.0A Supernatant Heat 70.0 ± 20.0B 25.0 ± 15A Supernatant Heat 90.0 ± 0B 45.0 ± 5.0A
Control   0 ± 0 Control   10 ± 3.3 Control   10 ± 3.3
  1. After twenty days of solid fermentation, rice was washed with Tween 80® (0.1% v/v). One part of crude extract was kept and the other part was centrifuged and filtered. Conidia were resuspended in Tween 80® (0.1% v/v) and supernatant stored. The three treatments (crude extract. conidia and supernatant) were separated into two subgroups: one kept at 27 ± 1 °C and the other exposed to 45 ± 0.2 °C for 2 h. Larvae were treated with the three types of treatments after biphasic fermentation in media supplemented (SUPPL) with riboflavin and sodium nitrate or unsupplemented media (UNSUPPL). The same treatments produced on different culture media or subjected to the same stress conditions were compared. Larvae were incubated at 25 ± 1 °C. Relative humidity greater than 75% and 12 h photoperiod for 10 days
  2. Equal capital letters in the same column indicate no significant difference according to Tukey's Test (P < 0.05)