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Table 6 Cumulative mortality of Aedes aegypti larvae after treatment with Metarhizium robertsii ARSEF 2575

From: Metarhizium robertsii protease and conidia production, response to heat stress and virulence against Aedes aegypti larvae

Treat Stress SUPPL UNSUPPL Treat Stress SUPPL UNSUPPL Treat Stress SUPPL UNSUPPL
Mortality (%) Mortality (%) Mortality (%)
3 days 5 days 10 days
Crude extract None 66.7 ± 8.8Aa 80.0 ± 5.8Aa Crude extract None 90.0 ± 5.8Aa 96.7 ± 3.3Aa Crude extract None 100 ± 0Aa 100.0 ± 0Aa
Conidia None 60.0 ± 5.8Aa 66.7 ± 12.0Aa Conidia None 80.0 ± 100 Aa 90.0 ± 5.8Aa Conidia None 100 ± 0Aa 96.7 ± 3.3Aa
Supernatant None 15.0 ± 5.0Ab 15.0 ± 5.0Ab Supernatant None 15.0 ± 5.0Ab 20.0 ± 0Ab Supernatant None 30.0 ± 10.0Ab 35.0 ± 5.0Ab
Crude extract Heat 60.0 ± 5.8Aa 50.0 ± 5.8Aab Crude extract Heat 76.0 ± 3.3Aa 83.3 ± 120 Aa Crude extract Heat 93.3 ± 3.3Aa 96.7 ± 3.3Aa
Conidia Heat 53.3 ± 3.33Aa 66.7 ± 6.7Aa Conidia Heat 83.3 ± 6.7Aa 90.0 ± 5.8Aa Conidia Heat 100 ± 0Aa 96.7 ± 3.3Aa
Supernatant Heat 70.0 ± 20.0Aa 15.0 ± 15.0Bb Supernatant Heat 70.0 ± 20.0Aa 25.0 ± 15Bb Supernatant Heat 90.0 ± 0Aa 45.0 ± 5.0Bb
Control   0 ± 0 Control   10 ± 3.3 Control   10 ± 3.3
  1. After twenty days of solid fermentation, rice was washed with Tween 80® (0.1% v/v). One part of crude extract was kept and the other part was centrifuged and filtered. Conidia were resuspended in Tween 80® (0.1% v/v) and supernatant stored. The three treatments (crude extract. conidia and supernatant) were separated into two subgroups: one kept at 27 ± 1 °C and the other exposed to 45 ± 0.2 °C for 2 h. Larvae were treated with the three types of treatments after biphasic fermentation in media supplemented (SUPPL) with riboflavin and sodium nitrate or unsupplemented media (UNSUPPL). The same treatments produced in different culture media or different treatments submitted to the same stress conditions were compared. Larvae were incubated at 25 ± 1 °C. Relative humidity greater than 75% and 12 h photoperiod for 10 days
  2. Equal capital letters in the same row indicate no significant difference and equal lower-case letters in the same column indicate no significant difference according to Tukey's test (P < 0.05)