Skip to main content

Table 4 Experimental design with 12 trials used to perform the response surface methodology

From: Metarhizium robertsii protease and conidia production, response to heat stress and virulence against Aedes aegypti larvae

Races

Riboflavin (%)

NaNO3 (%)

Proteolytic activity (U/mL)

Conidia/mL (10 9)

1

0.001

0.006

14.050

10.350

2

0.199

0.006

4.150

7.300

3

0.001

0.310

10.633

16.30

4

0.199

0.310

5.400

4.30

5

0

0.158

16.750

17.90

6

0.240

0.158

12.450

13.350

7

0.1

0

4.700

9.150

8

0.1

0.373

2.700

6.450

9

0.1

0.158

9.4

10.850

10

0.1

0.158

14.683

12.950

11

0.1

0.158

12.95

15.800

12

0.1

0.158

14.933

15.050

  1. Two variables were used (riboflavin and sodium nitrate) each with 5 levels and their respective values of proteolytic activity and conidia production of Metarhizium robertsii ARSEF 2575