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Table 4 Experimental design with 12 trials used to perform the response surface methodology

From: Metarhizium robertsii protease and conidia production, response to heat stress and virulence against Aedes aegypti larvae

Races Riboflavin (%) NaNO3 (%) Proteolytic activity (U/mL) Conidia/mL (10 9)
1 0.001 0.006 14.050 10.350
2 0.199 0.006 4.150 7.300
3 0.001 0.310 10.633 16.30
4 0.199 0.310 5.400 4.30
5 0 0.158 16.750 17.90
6 0.240 0.158 12.450 13.350
7 0.1 0 4.700 9.150
8 0.1 0.373 2.700 6.450
9 0.1 0.158 9.4 10.850
10 0.1 0.158 14.683 12.950
11 0.1 0.158 12.95 15.800
12 0.1 0.158 14.933 15.050
  1. Two variables were used (riboflavin and sodium nitrate) each with 5 levels and their respective values of proteolytic activity and conidia production of Metarhizium robertsii ARSEF 2575