Skip to main content

Table 3 Protease activity of Metarhizium robertsii ARSEF 2575 produced in medium supplemented with different inorganic salts and solid fermentation time

From: Metarhizium robertsii protease and conidia production, response to heat stress and virulence against Aedes aegypti larvae

Treatment Protease activity (U/mL)
Fermentation—10 dias
Protease activity (U/mL)
Fermentation—20 dias
Ammonium nitrate 10.7 ± 7.1ac 8.21 ± 4.4ab
Ammonium chloride 9.70 ± 4.0a 6.14 ± 0.8a
Potassium nitrate 19.7 ± 0.2ac 3.32 ± 0.3a*
Sodium nitrate 12.1 ± 0.1bc 23.1 ± 7.9b
Ammonium sulfate 11.4 ± 3.3ac 4.43 ± 1.8a*
Phosphorus nitrate 15.9 ± 1.4ac 9.16 ± 2.1ab
Urea 7.63 ± 0.1a 5.23 ± 1.6a
Negative control 12.9 ± 0.8ac 3.72 ± 1.2a
Positive control 14.8 ± 5.1ac 1.92 ± 1.3a*
  1. Protease essay was evaluated after biphasic fermentation with solid fermentation in parboiled rice for 10 or 20 days
  2. Lowercase letters refer to comparison between different components in culture medium, where averages followed by equal letters in the same column do not differ significantly from each other (P > 0.05)
  3. *Refer to comparison between different components in the culture medium, where averages in the same column differ significantly in relation to the group supplemented with sodium nitrate (P > 0.01)