Metarhizium robertsii protease and conidia production, response to heat stress and virulence against Aedes aegypti larvae

AMB Express

Table 3 Protease activity of Metarhizium robertsii ARSEF 2575 produced in medium supplemented with different inorganic salts and solid fermentation time

Treatment	Protease activity (U/mL) Fermentation—10 dias	Protease activity (U/mL) Fermentation—20 dias
Ammonium nitrate	10.7 ± 7.1^ac	8.21 ± 4.4^ab
Ammonium chloride	9.70 ± 4.0^a	6.14 ± 0.8^a
Potassium nitrate	19.7 ± 0.2^ac	3.32 ± 0.3^a*
Sodium nitrate	12.1 ± 0.1^bc	23.1 ± 7.9^b
Ammonium sulfate	11.4 ± 3.3^ac	4.43 ± 1.8^a*
Phosphorus nitrate	15.9 ± 1.4^ac	9.16 ± 2.1^ab
Urea	7.63 ± 0.1^a	5.23 ± 1.6^a
Negative control	12.9 ± 0.8^ac	3.72 ± 1.2^a
Positive control	14.8 ± 5.1^ac	1.92 ± 1.3^a*

Protease essay was evaluated after biphasic fermentation with solid fermentation in parboiled rice for 10 or 20 days
Lowercase letters refer to comparison between different components in culture medium, where averages followed by equal letters in the same column do not differ significantly from each other (P > 0.05)
*Refer to comparison between different components in the culture medium, where averages in the same column differ significantly in relation to the group supplemented with sodium nitrate (P > 0.01)