Fig. 3

Comparison between relative viability (%) of Metarhizium robertsii ARSEF 2575 produced in parboiled rice supplemented and unsupplemented with riboflavin and sodium nitrate after exposure to heat stress. Rice was washed with Tween 80® (0.01% v/v) after twenty days of solid fermentation. One part of the crude extract was kept and the other part was centrifugates, filtered and conidia were resuspended in Tween 80® (0.01% v/v). Samples of each group were exposed to 45 ± 0.2 °C and at each 1 h of exposure an aliquot was inoculated in culture medium for germination analysis. Different treatments were compared at the same exposure times: A: Crude extract produced in supplemented and unsupplemented medium; B: Conidia produced in supplemented and unsupplemented medium; C: Crude extract and conidia produced in supplemented medium; D: Crude extract and conidia produced in unsupplemented medium. Samples of each group were exposed to 45 ± 0.2 °C and at each 1 h of exposure an aliquot was inoculated in culture medium for germination analysis. Different letters indicates *P < 0.05 according to Tukey’s Test