Skip to main content
Fig. 5 | AMB Express

Fig. 5

From: Evaluation of P450 monooxygenase activity in lyophilized recombinant E. coli cells compared to resting cells

Fig. 5

A Influence of cofactor regeneration by Re-ADH in E. coli C43 (DE3) pET22b-cyp105D + pCOLADuet- pdx-pdr-adh on testosterone 1 conversion and formation of 2β-hydroxytestosterone 2 (2β-OH-Tes). B Effect of different ratios of propan-2-ol and acetone on testosterone 1 conversion and formation of 2β-OH-Tes mediated by the wet cells without ADH (P450 + redox partners). The best performing wet cell biocatalyst (`frozen as cell pellet’) was investigated (see Fig. 3). Reaction conditions: 10 mg/mL lyophilized cells or 50 mg/mL wet cells in 0.5 mL Phosphate Sucrose EDTA (PSE)-buffer with 1 × nutrient solution, pH 7.5 in 2 mL reaction tubes; 1 mM testosterone 1 dissolved in 5% co-solvent (v/v) final concentration, 25 °C, 1100 min−1 shaking frequency, reaction time 20 h. All measurements were performed in technical duplicates. In case a standard deviation is given, experiments were additionally conducted in biological duplicates

Back to article page